The Arabidopsis selenium-binding protein confers tolerance to toxic levels of selenium

Functional Plant Biology : FPB
Adamantia AgalouHerman P Spaink

Abstract

In the Arabidopsis genome there are three highly conserved homologues of the mammalian 56-kD selenium-binding protein (SBP). To study the function of SBP in this model plant, we used a transgenic approach by constitutively overexpressing and down-regulating the endogenous Atsbp1 gene. In the latter case, we employed both a conventional antisense method and gene silencing by intron-containing hairpin RNAs. Atsbp1-overexpressing and silenced plants were phenotypically normal, under standard growth conditions, when compared with wild type plants. Transgenic plants exhibited different growth responses to exogenously supplied selenite, which correlated with the expression levels of Atsbp1. Plants with increased Atsbp1 transcript levels showed enhanced tolerance to selenite, while plants with reduced levels were more sensitive. Our results indicate that, although Atsbp1 does not play a detectable role in the regulation of developmental processes under normal growth conditions, it appears to be involved in processes controlling tolerance of Arabidopsis to selenium toxicity.

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Citations

Mar 17, 2006·The New Phytologist·Lihong ZhangElizabeth A H Pilon-Smits
Jan 1, 2016·Annals of Botany·Philip J White
Feb 11, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Akbar HossainAlison M Laing
Oct 18, 2020·Journal of Hazardous Materials·Richard C Trippe, Elizabeth A H Pilon-Smits
Aug 28, 2020·Plant Physiology and Biochemistry : PPB·Salvador González-GordoFrancisco J Corpas
Aug 21, 2019·Plant Physiology and Biochemistry : PPB·Akyla Maria Martins AlvesFabienne Micheli

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Methods Mentioned

BETA
environmental stress
transgenic
PCR

Software Mentioned

BLAST

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