The Arabidopsis thaliana-Alternaria brassicicola pathosystem: A model interaction for investigating seed transmission of necrotrophic fungi.

Plant Methods
Stéphanie PochonClaire Campion

Abstract

Seed transmission constitutes a major component of the parasitic cycle for several fungal pathogens. However, very little is known concerning fungal or plant genetic factors that impact seed transmission and mechanisms underlying this key biological trait have yet to be clarified. Such lack of available data could be probably explained by the absence of suitable model pathosystem to study plant-fungus interactions during the plant reproductive phase. Here we report on setting up a new pathosystem that could facilitate the study of fungal seed transmission. Reproductive organs of Arabidopsis thaliana were inoculated with Alternaria brassicicola conidia. Parameters (floral vs fruit route, seed collection date, plant and silique developmental stages) that could influence the seed transmission efficiency were tested to define optimal seed infection conditions. Microscopic observations revealed that the fungus penetrates siliques through cellular junctions, replum and stomata, and into seed coats either directly or through cracks. The ability of the osmosensitive fungal mutant nik1Δ3 to transmit to A. thaliana seeds was analyzed. A significant decrease in seed transmission rate was observed compared to the wild-type parental strain,...Continue Reading

References

Nov 1, 1995·The Plant Journal : for Cell and Molecular Biology·B W ShirleyH M Goodman
Jan 6, 2006·Planta·Jean-Marc RoutaboulLoïc Lepiniec
Dec 13, 2006·Trends in Plant Science·Lucille PourcelIsabelle Debeaujon
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Mar 4, 2011·Current Drug Targets·Nikolaus L Schlaich

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Citations

Nov 25, 2014·Fungal Biology Reviews·Karthik Krishnan, David S Askew
Jul 15, 2015·Environmental Microbiology·Stephanie KlaedtkeMatthieu Barret
Nov 6, 2013·Virulence·Thomas GuillemettePhilippe Simoneau
Feb 5, 2019·Journal of Experimental Botany·Kay GullySébastien Aubourg
Feb 14, 2018·Genome Announcements·Elodie BelmasThomas Guillemette
Sep 24, 2019·Frontiers in Microbiology·Guillaume Quang N'GuyenThomas Guillemette

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Methods Mentioned

BETA
scanning electron microscopy
transmission electronic microscopy

Software Mentioned

R

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