The archaeal sRNA binding protein L7Ae has a 3D structure very similar to that of its eukaryal counterpart while having a broader RNA-binding specificity

Journal of Molecular Biology
Christophe CharronA Aubry

Abstract

The ribosomal L7Ae protein of archaea has the peculiarity to be a component of the C/D and H/ACA snRNPs, that guide rRNA post-transcriptional modifications. Its yeast (Snu13p) and human (15.5kDa protein) homologs are only found in C/D snoRNPs and the (U4/U6, U5) spliceosomal tri-snRNP. By using a large variety of RNAs, we compared the RNA-binding specificities of the recombinant Pyrococcus abyssi L7Ae and Saccharomyces cerevisiae Snu13 proteins. Unlike Snu13p, protein L7Ae binds terminal loops closed by two A:G and G:A pairs and canonical K-turn structures with similar efficiencies, provided that the terminal loop contains at least 5nt. In contrast to Snu13p, binding of protein L7Ae to canonical K-turn structures is not dependent on the identity of the residue at position 2 in the bulge. The peculiar KT-15 motif of P. abyssi 23S rRNA, that is recognized by L7Ae, does not associate with Snu13p. To get more information on the P. abyssi L7Ae protein, we solved its X-ray structure at 1.9A resolution. In spite of their sequence divergence, the free P. abyssi and bound H. marismortui proteins were found to have highly similar structures. Only a limited number of side-chain conformational changes occur at the protein-RNA interface upo...Continue Reading

References

Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Jun 22, 1999·Journal of Molecular Biology·L Xiao, B Honig
Sep 24, 1999·Journal of Molecular Biology·H Mao, J R Williamson
Dec 3, 1999·Nature Structural Biology·A D Frankel
Mar 25, 2000·Molecular and Cellular Biology·D L Lafontaine, D Tollervey
Apr 25, 2000·Science·A D OmerP P Dennis
Aug 3, 2001·The EMBO Journal·D J KleinT A Steitz
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Arina D OmerPatrick P Dennis
Sep 7, 2002·Molecular and Cellular Biology·Silvia GalardiIrene Bozzoni
Oct 29, 2002·Chemistry & Biology·Lara B Weinstein SzewczakJoan A Steitz
Nov 30, 2002·Biochimie·Jean Pierre BachellerieAlexander Hüttenhofer
Feb 19, 2003·Journal of Molecular Biology·Jeffrey A ChaoJames R Williamson
Feb 25, 2003·Nature Structural Biology·Mohamed AittalebHong Li
Apr 16, 2003·Molecular Microbiology·Arina D OmerPatrick P Dennis
Nov 7, 2003·Nucleic Acids Research·Marie-Line BortolinBéatrice Clouet-d'Orval
Dec 20, 2003·Acta Crystallographica. Section D, Biological Crystallography·Christophe CharronAndré Aubry

❮ Previous
Next ❯

Citations

Jun 21, 2005·Biochemical and Biophysical Research Communications·Sri vdya OrugantiHong Li
Apr 12, 2005·Nature Structural & Molecular Biology·Laurent ChavatteDonna M Driscoll
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Keqiong YeLiman Zhang
Aug 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·I-Ming ChoVenkat Gopalan
Jun 4, 2005·Nucleic Acids Research·Bruno CharpentierChristiane Branlant
Jun 16, 2005·Nucleic Acids Research·Vlad CojocaruThomas M Jovin
Nov 19, 2005·Nucleic Acids Research·Sophie NolivosBéatrice Clouet-d'Orval
Feb 8, 2007·Nucleic Acids Research·Steve L ReichowGabriele Varani
May 5, 2005·Genes & Development·Daniel L BakerMichael P Terns
May 10, 2013·Annual Review of Biophysics·Alfonso Mondragón
Jul 31, 2014·Nucleic Acids Research·Gabrielle Deschamps-FrancoeurMichelle S Scott
Jan 5, 2016·Nature Chemical Biology·Xuesong ShiDaniel Herschlag
Jul 7, 2007·Journal of Molecular Biology·Nicolas LeulliotHerman Van Tilbeurgh
Nov 19, 2014·The Journal of Cell Biology·Jonathan BizarroEdouard Bertrand
Nov 2, 2014·Nucleic Acids Research·Stella M LaiVenkat Gopalan
Apr 23, 2016·Biomolecules·Manoj P SamantaVenkat Gopalan
Jun 28, 2016·Nucleic Acids Research·W S Vincent YipSusan J Baserga
Sep 30, 2016·Biochemistry. Biokhimii︠a︡·M Y ShubinaE V Sheval
Aug 31, 2013·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Mohammad Wadud BhuiyaBernard Andrew Brown
Apr 24, 2018·FEMS Microbiology Reviews·Béatrice Clouet-d'OrvalLisa-Katharina Maier
Jun 3, 2021·Non-coding RNA·Laeya BaldiniStéphane Labialle

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.