Oct 29, 2018

The architecture of cell differentiation in choanoflagellates and sponge choanocytes

BioRxiv : the Preprint Server for Biology
Davis LaundonPawel Burkhardt

Abstract

Collar cells are ancient animal cell types which are conserved across the animal kingdom and their closest relatives, the choanoflagellates. However, little is known about their ancestry, their subcellular architecture, or how they differentiate. The choanoflagellate Salpingoeca rosetta expresses genes necessary for animal multicellularity and development and can alternate between unicellular and multicellular states making it a powerful model to investigate the origin of animal multicellularity and mechanisms underlying cell differentiation. To compare the subcellular architecture of solitary collar cells in S. rosetta with that of multicellular 'rosettes' and collar cells in sponges, we reconstructed entire cells in 3D through transmission electron microscopy on serial ultrathin sections. Structural analysis of our 3D reconstructions revealed important differences between single and colonial choanoflagellate cells, with colonial cells exhibiting a more amoeboid morphology consistent with relatively high levels of macropinocytotic activity. Comparison of multiple reconstructed rosette colonies highlighted the variable nature of cell sizes, cell-cell contact networks and colony arrangement. Importantly, we uncovered the presenc...Continue Reading

  • References
  • Citations

References

  • We're still populating references for this paper, please check back later.
  • References
  • Citations

Citations

  • This paper may not have been cited yet.

Mentioned in this Paper

Asphondylia rosetta
Size
Unicellular Trichome Branch
Microvilli
Golgi Apparatus
Genes
Three-dimensional
Reconstructive Surgical Procedures
Science of Morphology
Morphological

About this Paper

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.