PMID: 9163597May 1, 1997Paper

The Arg633His substitution responsible for the private platelet antigen Gro(a) unravelled by SSCP analysis and direct sequencing

British Journal of Haematology
S SimsekA E von dem Borne

Abstract

We have previously described the private or family platelet antigen, Gro(a), which was identified in a case of neonatal alloimmune thrombocytopenia. The Gro(a) antigen was found to be located on the GP IIIa (beta3) subunit of the GP IIb/IIIa complex, the most prominent fibrinogen receptor of platelets. Initial experiments to characterize the Gro(a) antigen at the molecular genetic level were unsuccessful. We therefore decided to use a different strategy to unravel the molecular basis of this antigen. Platelet GP IIIa mRNA of a Gro(a+) and a Gro(a-) donor was amplified with suitable primers in a reverse transcriptase-polymerase chain reaction (RT-PCR) and subjected to single-strand conformational polymorphism (SSCP) analysis. Three regions of the amplified GP IIIa cDNA derived from the Gro(a+) donor showed a different SSCP pattern when compared to that of the Gro(a-) donor. Direct nucleotide sequence analysis of these three segments revealed that two of them contained silent substitutions, A1163C, A1553G and G1565A. The first and the latter changes were described previously. In the third segment a G1996A mutation was found, predicting an arginine --> histidine substitution at position 633 of the mature glycoprotein. PCR-ASRA (al...Continue Reading

Citations

May 3, 2003·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Sentot Santoso
Aug 15, 1998·Vox Sanguinis·S Santoso, V Kiefel
Aug 15, 1998·Vox Sanguinis·H KrollS Santoso
Dec 17, 2003·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·D C JonesS E Marshall
Feb 7, 2013·British Journal of Haematology·Julie A PetersonRichard H Aster
May 5, 2009·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·G Bertrand, C Kaplan
Apr 2, 2014·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Barbera VeldhuisenMasja de Haas
Dec 15, 2016·Transfusion Medicine Reviews·Guoguang WuHeyu Ni
Jun 25, 2009·Vox Sanguinis·B VeldhuisenM de Haas

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