The aromatase inhibitor fadrozole and the 5-reductase inhibitor finasteride affect gonadal differentiation and gene expression in the frog Silurana tropicalis

Sexual Development : Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation
P Duarte-GutermanV L Trudeau

Abstract

Aromatase (cyp19) and the 5alpha- and 5beta-reductases (srd5alpha and srd5beta) are important enzymes for vertebrate sexual development. We investigated the effects of inhibition of cyp19 by fadrozole (FAD), and srd5alpha and srd5beta by finasteride (FIN) during anuran larval development. Chronic exposures of Silurana (Xenopus) tropicalis from Nieuwkoop-Faber stage 12 until stage 60 were performed using either 2 microM FAD or 25 microM FIN. Histological analysis of exposed metamorphic frogs revealed that both treatments induced intersex individuals (presence of testicular oocytes). FAD treatment resulted in 55% male, 30% female and 15% intersex, while FIN treatment produced 27% male, 53% female and 20% intersex. Real-time RT-PCR analysis of hepatic sex steroid- and thyroid hormone-related gene expression demonstrated that FAD-induced intersex animals had higher srd5alpha1, srd5alpha2 and eralpha mRNA levels than control and FAD males. In contrast, FIN-induced intersex had low srd5alpha1, srd5alpha2, srd5beta and dio3 and high dio2 mRNA levels. FIN-treated males exhibited high trbeta, dio2 and a lower dio3 mRNA levels. We conclude that chemically induced intersex animals display different gene expression profiles than non-expose...Continue Reading

Citations

Oct 8, 2013·General and Comparative Endocrinology·Christopher J MartyniukValérie S Langlois
Jan 29, 2011·Journal of Toxicology and Environmental Health. Part a·Melissa A DavidsonDavid R S Lean
Apr 15, 2010·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Dapeng ZhangVance L Trudeau
Oct 23, 2014·Biological Reviews of the Cambridge Philosophical Society·Frances Orton, Charles R Tyler
Feb 18, 2014·General and Comparative Endocrinology·Sonja BisseggerValérie S Langlois
Apr 2, 2014·General and Comparative Endocrinology·Paula Duarte-GutermanVance L Trudeau
Jun 24, 2014·General and Comparative Endocrinology·Christopher J MartyniukValérie S Langlois
Apr 10, 2014·General and Comparative Endocrinology·Diana E K Flood, Valerie S Langlois
Sep 8, 2015·General and Comparative Endocrinology·Anna OrnostayChristopher J Martyniuk
Feb 11, 2015·General and Comparative Endocrinology·Justine Mathieu-DenoncourtValerie S Langlois
Apr 26, 2015·General and Comparative Endocrinology·Michael R LeeKenneth Oliveira
Jul 5, 2016·Mechanisms of Development·Sonja Bissegger, Valerie S Langlois
Jan 8, 2011·Sexual Development : Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation·V S LangloisV L Trudeau
Sep 21, 2016·Sexual Development : Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation·Stéphane Flament
Mar 16, 2018·International Journal of Molecular Sciences·Carmen J Zamora-SánchezIgnacio Camacho-Arroyo
Feb 27, 2020·Cell Biology International·Juliana S MaldarineSebastião R Taboga
Aug 28, 2019·Environmental Toxicology·Juliana S MaldarineSebastião R Taboga
Sep 13, 2017·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Cheryl S RosenfeldVance L Trudeau
Jan 26, 2020·General and Comparative Endocrinology·Julie Robitaille, Valerie S Langlois
May 26, 2020·Molecular and Cellular Endocrinology·Vance L TrudeauValérie S Langlois

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