PMID: 8096Jun 15, 1976

The aromatic and heme chromophores of rabbit hemopexin. Difference absorption and fluorescence spectra

Biochimica Et Biophysica Acta
W T MorganU Muller-eberhard

Abstract

Spectrophotometric and fluorimetric techniques were employed to charcterize the environment of the heme chromophore of rabbit hemopexin and to monitor changes in the environment of aromatic amino acid residues induced by the interaction of hemopexin with porphyrins and metalloporphyrins. Difference spectra showed maxima at 292 and 285 nm when hemopexin binds heme or deuteroheme but not deuteroporphyrin. These maxima are attributed to alterations in the local environment of tryptophan and tyrosine residues. Spectro-photometric titrations of the tyrosine residues of hemopexin, heme-hemopexin and hemopexin in 8 M urea showed apparent pK values at 11.4, 11.7, and 10.9 respectively. Perturbation difference spectra produced by 20% v/v ethylene glycol are consistent with the exposure of 6-8 of the 14 tyrosine residues and 6-8 of the 15 tryptophan residues of rabbit hemopexin to this perturbant. Only small differences were found between the perturbation spectra of apo- and heme-hemopexin near 290 nm, suggesting that slight or compensating changes in the exposure to solvent of tryptophan chromophores occur. In the Soret spectral region, the exposure of heme in the heme-hemopexin complex to ethylene glycol was 0.7, relative to the fully ...Continue Reading

References

Feb 1, 1975·Proceedings of the Society for Experimental Biology and Medicine·H H LiemU Muller-eberhard
May 1, 1972·Archives of Biochemistry and Biophysics·V L SeeryU M Eberhard
Oct 22, 1974·Biochemistry·E M Brown, R M Parry
Apr 11, 1974·The New England Journal of Medicine·R D WochnerU Muller-eberhard
Apr 11, 1974·Biochimica Et Biophysica Acta·T G Bukolova-Orlova Yukelson LYa
Feb 10, 2009·Journal of Nanoscience and Nanotechnology·P PantM H Manghnani

Citations

Jan 1, 1995·Protein Science : a Publication of the Protein Society·M L Wu, W T Morgan
Feb 1, 1991·Journal of Protein Chemistry·P MusterW T Morgan
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·Y Leutzinger, S Beychok
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·N TakahashiF W Putnam
Sep 24, 1985·Biochemistry·R F PasternackU Muller-eberhard
Oct 1, 1976·Archives of Biochemistry and Biophysics·W T Morgan, U Muller-eberhard
Jun 15, 1977·FEBS Letters·Y PlanckeG Biserte
May 1, 2010·The Journal of Biological Chemistry·Marcia R Mauk, A Grant Mauk

Related Concepts

Metazoa
Apoproteins
Heme
Hemopexin
Hydrogen Bonding
Hydrogen-Ion Concentration
Plasma Protein Binding Capacity
Protein Conformation
Chinchilla Rabbits
Fluorescence Spectroscopy

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