The arrest gene is required for germline cyst formation during Drosophila oogenesis

Genesis : the Journal of Genetics and Development
M J ParisiH Lin

Abstract

In Drosophila, oogenesis is initiated when a germline stem cell produces a differentiating daughter cell called the cystoblast. The cystoblast undergoes four rounds of synchronous divisions with incomplete cytokinesis to generate a syncytial cyst of 16 interconnected cystocytes, in which one cystocyte differentiates into an oocyte. Strong mutations of the arrest (aret) gene disrupt cyst formation and cause the production of clusters of ill-differentiated germline cells that retain cellular and molecular characteristics of cystoblasts. These mutant germ cells express high levels of BAM-C and SXL proteins in the cytoplasm but do not accumulate markers for advanced cystocytes or differentiating oocytes, such as the nuclear localization of SXL or the accumulation of osk mRNA, orb mRNA, and cytoplasmic dynein. However, the mutant germ cells do not contain spectrosomes, the cytoplasmic structure that objectifies the divisional asymmetry of the cystoblast. The aret mutant germ cells undergo active mitosis with complete cytokinesis. Their mitosis is accompanied by massive necrosis, so that the number of germ cells in a stem cell-derived cluster ranges from one to greater than 70. These defects of aret mutants reveal a novel function of...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Dec 1, 1990·Genes & Development·D M McKearin, A C Spradling
Jan 1, 1995·Developmental Genetics·H Lin, A C Spradling
May 1, 1996·Zygote : the Biology of Gametes and Early Embryos·M Zaccai, H D Lipshitz
Jan 1, 1996·Annual Review of Cell and Developmental Biology·A Williamson, R Lehmann
Jul 10, 1997·Nature·T Lecuit, S M Cohen
Jan 1, 1997·Annual Review of Genetics·M de CuevasA C Spradling
Dec 10, 1998·Nucleic Acids Research·UNKNOWN FlyBase Consortium
Jan 23, 1999·Current Opinion in Cell Biology·H Lin

❮ Previous
Next ❯

Citations

Mar 9, 2007·Development·Jean Z MainesDennis M McKearin
Aug 8, 2006·Developmental Cell·Tingxia GuoPhillip A Newmark
Feb 20, 2003·Mechanisms of Development·Paolo Filardo, Anne Ephrussi
Apr 28, 2009·Mechanisms of Development·Jocelyn MoorePaul Lasko
Jul 17, 2010·Developmental Biology·Lori Dawn Horb, Marko E Horb

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.