The artificial loss of Runx1 reduces the expression of quiescence-associated transcription factors in CD4(+) T lymphocytes

Molecular Immunology
Won Fen WongMasanobu Satake

Abstract

The Runx1 transcription factor cooperates with or antagonizes other transcription factors and plays essential roles in the differentiation and function of T lymphocytes. Previous works showed that Runx1 is expressed in peripheral CD4(+) T cells which level declines after T cell receptor (TCR) activation, and artificial deletion of Runx1 causes autoimmune lung disease in mice. The present study addresses the mechanisms by which Runx1 contributes to the maintenance of peripheral CD4(+) T cell quiescence. Microarray and quantitative RT-PCR analyses were employed to compare the transcriptome of Runx1 -/- CD4(+) T cells to those of unstimulated and TCR-stimulated Runx1 +/- cells. The results identified genes whose expression was modulated similarly by Runx1 deletion and TCR activation. Among them, genes encoding cytokines, chemokines, and Jak/STAT signaling molecules were substantially induced. In Runx1-deleted T cells, simultaneous increases in Il-17A and Rorγc, a known master gene in TH17 differentiation, were observed. In addition, we observed that the loss of Runx1 reduced the transcription of genes encoding quiescence-associated transcription factors, including Foxp1, Foxo1, and Klf2. Interestingly, we identified consensus Runx...Continue Reading

References

Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A StrasserA W Harris
Jun 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Eilon WoolfYoram Groner
May 25, 2004·Oncogene·Ditsa Levanon, Yoram Groner
Feb 3, 2005·Oncogene·Lindsay C SpenderPaul J Farrell
May 3, 2005·Nature Reviews. Cancer·Karen BlythJames C Neil
Jul 25, 2007·The Journal of Experimental Medicine·Takeshi EgawaDan R Littman
Sep 17, 2008·The Journal of Experimental Medicine·Mark GilchristAlan Aderem
Jul 16, 2009·Journal of Cellular Physiology·Gareth Brady, Paul J Farrell
Feb 11, 2010·Blood Cells, Molecules & Diseases·Chelsia Qiuxia WangMotomi Osato
Aug 17, 2010·The International Journal of Developmental Biology·Gemma SwiersNancy A Speck
Nov 16, 2013·Cytokine & Growth Factor Reviews·Francesca B AielloScott K Durum
Dec 7, 2013·European Journal of Immunology·Won Fen WongKazuyoshi Kohu

❮ Previous
Next ❯

Citations

Oct 21, 2020·Nature Reviews. Immunology·Mohamed A ElTanbouly, Randolph J Noelle
Aug 24, 2021·Frontiers in Immunology·Svetlana KorinfskayaArtem Barski

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Atlas of the Human Thymus

Examining the genomics, transcriptomics, proteomics, and metabolomics of the human thymus gland at the single cell level will help build a unique profile for each cell type and a three-dimensional map of how cell types work together to form tissues. Discover the latest research on the cell atlas of the human thymus here.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.