PMID: 7035166Dec 1, 1981Paper

The aryl acylamidases and their relationship to cholinesterases in human serum, erythrocyte and liver

European Journal of Biochemistry
S T George, A S Balasubramanian

Abstract

Human serum aryl acylamidase associated with serum cholinesterase was purified to homogeneity. Evidence for the identity of the two enzymes was based on co-elution profiles, co-purification in the different steps including affinity chromatography with constant ratios of specific activity and percentage recoveries, co-migration on gel electrophoresis, parallel inhibition by typical cholinesterase inhibitors and co-precipitation by antibody raised against the purified enzyme. Human liver aryl acylamidase was partially purified. Based on the elution profiles, purification data, inhibitory characteristics and gel electrophoresis it was concluded that aryl acrylamidase of liver was not associated with liver cholinesterase. More conclusive evidence for the non-association of the liver aryl acylamidase and cholinesterase came from their clear-cut separation on procainamide-Sepharose affinity chromatography. Both the serum and liver aryl acylamidase were compared with the purified erythrocyte aryl acylamidase associated with acetylcholinesterase. While the erythrocyte and serum aryl acylamidases showed some similarities in their sensitivities to amines like serotonin or tryptamine and choline derivatives, the liver enzyme was unaffecte...Continue Reading

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Citations

Jan 15, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·S T GeorgeA S Balasubramanian
Aug 15, 1989·Clinica Chimica Acta; International Journal of Clinical Chemistry·R V RaoA S Balasubramanian
Feb 14, 1992·Clinica Chimica Acta; International Journal of Clinical Chemistry·L D JayanthiA S Balasubramanian
Aug 8, 1998·The International Journal of Biochemistry & Cell Biology·C D Bhanumathy, A S Balasubramanian
Jun 24, 2011·Chemical Reviews·Jonathan Z Long, Benjamin F Cravatt
Jan 13, 2000·Protein Science : a Publication of the Protein Society·R ArnonR Tarrab-Hazdai
Jan 2, 1987·European Journal of Biochemistry·R Boopathy, A S Balasubramanian
Jul 1, 1991·European Journal of Biochemistry·H YoshiokaH Yamada
Oct 3, 2012·PloS One·María-Salud García-AyllónJavier Sáez-Valero
Jan 23, 2008·Biological Chemistry·María F MontenegroCecilio J Vidal
Apr 20, 2010·Chemico-biological Interactions·Gracielle Alves-AmaralRosely Oliveira Godinho
Oct 24, 2009·Journal of Pharmaceutical and Biomedical Analysis·Matthias KoitkaHans-Hubert Borchert
Aug 11, 2007·Biochimica Et Biophysica Acta·Patrick MassonLawrence M Schopfer
Nov 15, 1984·Analytical Biochemistry·P M HammondC P Price
Mar 24, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Rathanam BoopathyPaul G Layer
Jul 18, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rona R Ramsay, Keith F Tipton
Feb 4, 2003·Nature Reviews. Neuroscience·Sultan DarveshChangiz Geula
Feb 24, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M Salud García-AyllónJavier Sáez-Valero
Jun 10, 2003·Alzheimer Disease and Associated Disorders·Sultan DarveshEarl Martin
Sep 13, 2007·Journal of Enzyme Inhibition and Medicinal Chemistry·Patrick MassonOksana Lockridge
May 26, 2018·Environmental Science and Pollution Research International·Caio R D AssisLuiz B Carvalho
Dec 12, 2020·Progress in Biophysics and Molecular Biology·Israel Silman

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