The ascent of man(made oxidoreductases)

Current Opinion in Structural Biology
Katie J Grayson, Jl Ross Anderson

Abstract

Though established 40 years ago, the field of de novo protein design has recently come of age, with new designs exhibiting an unprecedented level of sophistication in structure and function. With respect to catalysis, de novo enzymes promise to revolutionise the industrial production of useful chemicals and materials, while providing new biomolecules as plug-and-play components in the metabolic pathways of living cells. To this end, there are now de novo metalloenzymes that are assembled in vivo, including the recently reported C45 maquette, which can catalyse a variety of substrate oxidations with efficiencies rivalling those of closely related natural enzymes. Here we explore the successful design of this de novo enzyme, which was designed to minimise the undesirable complexity of natural proteins using a minimalistic bottom-up approach.

Citations

Feb 12, 2020·Quarterly Reviews of Biophysics·Ivan V Korendovych, William F DeGrado
Jul 14, 2020·Biotechnology and Applied Biochemistry·Linda LeoneAngela Lombardi
Sep 27, 2018·International Journal of Molecular Sciences·Gerardo ZambranoAngela Lombardi
Apr 12, 2020·Biotechnology and Applied Biochemistry·Richard Stenner, John Leslie Ross Anderson
Aug 31, 2018·Journal of the Royal Society, Interface·Katie J Grayson, J L Ross Anderson
Jan 6, 2021·Chemical Communications : Chem Comm·Ornella MaglioAngela Lombardi
Feb 4, 2021·Biochemistry·Areetha D'Souza, Surajit Bhattacharjya
Jan 20, 2021·Biotechnology and Applied Biochemistry·Gerardo ZambranoFlavia Nastri
Feb 24, 2021·Journal of Inorganic Biochemistry·Jonathan M X JenkinsJ L Ross Anderson
Apr 18, 2021·International Journal of Biological Macromolecules·Yale Luck NunesJosé C S Dos Santos
May 3, 2021·Protein Science : a Publication of the Protein Society·José M PereiraSérgio M Santos
Jul 25, 2019·Current Opinion in Chemical Biology·William M DawsonDerek N Woolfson

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Methods Mentioned

BETA
protein folding

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