The ASH1 HOMOLOG 2 (ASHH2) histone H3 methyltransferase is required for ovule and anther development in Arabidopsis.

PloS One
Paul E GriniReidunn B Aalen

Abstract

SET-domain proteins are histone lysine (K) methyltransferases (HMTase) implicated in defining transcriptionally permissive or repressive chromatin. The Arabidopsis ASH1 HOMOLOG 2 (ASHH2) protein (also called SDG8, EFS and CCR1) has been suggested to methylate H3K4 and/or H3K36 and is similar to Drosophila ASH1, a positive maintainer of gene expression, and yeast Set2, a H3K36 HMTase. Mutation of the ASHH2 gene has pleiotropic developmental effects. Here we focus on the role of ASHH2 in plant reproduction. A slightly reduced transmission of the ashh2 allele in reciprocal crosses implied involvement in gametogenesis or gamete function. However, the main requirement of ASHH2 is sporophytic. On the female side, close to 80% of mature ovules lack embryo sac. On the male side, anthers frequently develop without pollen sacs or with specific defects in the tapetum layer, resulting in reduction in the number of functional pollen per anther by up to approximately 90%. In consistence with the phenotypic findings, an ASHH2 promoter-reporter gene was expressed at the site of megaspore mother cell formation as well as tapetum layers and pollen. ashh2 mutations also result in homeotic changes in floral organ identity. Transcriptional profilin...Continue Reading

References

May 1, 1969·Stain Technology·M P Alexander
Nov 14, 1997·The Plant Journal : for Cell and Molecular Biology·M G AartsA Pereira
Mar 6, 1998·Cellular and Molecular Life Sciences : CMLS·T JenuweinG Reuter
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·U SchiefthalerK Schneitz
Jun 30, 2001·Science·J A MayfieldD Preuss
Feb 19, 2002·The Journal of Biological Chemistry·Philip ZegermanTony Kouzarides
Nov 7, 2002·Cell·Bryan M Turner
Jan 22, 2003·The Plant Journal : for Cell and Molecular Biology·Anna-Marie SorensenHeinz Saedler
Apr 18, 2003·Current Biology : CB·Raul Alvarez-VenegasZoya Avramova
Oct 14, 2003·Plant Physiology·Mark D Curtis, Ueli Grossniklaus
Aug 13, 2005·The International Journal of Developmental Biology·Pedro Robles, Soraya Pelaz
Sep 17, 2005·Nature Methods·Anne-Valérie GendrelVincent Colot
Feb 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·Lidiya I SergeevaDick Vreugdenhil
May 2, 2006·Statistical Applications in Genetics and Molecular Biology·Gordon K Smyth
Aug 16, 2006·Journal of Experimental Botany·Gema Vizcay-Barrena, Zoe A Wilson
Feb 27, 2007·Cell·Tony Kouzarides
Jun 15, 2007·Biochemical and Biophysical Research Communications·Marianne StabellAndrew Lambertsson
Aug 22, 2007·Current Opinion in Plant Biology·E S Dennis, W J Peacock
Feb 7, 2008·Biochemical and Biophysical Research Communications·Hyun Soo KimYeun Kyu Jang
Jul 8, 2008·Biochemical and Biophysical Research Communications·Gaofeng DongJiaxu Li

❮ Previous
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Citations

Aug 19, 2014·Trends in Plant Science·Mieke Van Lijsebettens, Klaus D Grasser
Feb 5, 2011·Plant Cell Reports·Felipe AqueaPatricio Arce-Johnson
Jul 23, 2011·Biochimica Et Biophysica Acta·Alexandre BerrWen-Hui Shen
Jun 15, 2011·Biochimica Et Biophysica Acta·Tage ThorstensenReidunn Birgitta Aalen
Dec 6, 2011·The Plant Journal : for Cell and Molecular Biology·Pengfei SuiAiwu Dong
Jul 22, 2010·Development, Growth & Differentiation·Ming ChenYijun Meng
Oct 26, 2011·Molecular Plant Pathology·Clelia De-La-PeñaRaúl Alvarez-Venegas
Sep 10, 2013·Wiley Interdisciplinary Reviews. Developmental Biology·Swaminathan Venkatesh, Jerry L Workman
Apr 28, 2011·The EMBO Journal·Verena HoppmannRein Aasland
Mar 22, 2014·The Arabidopsis Book·Dong-Hwan Kim, Sibum Sung
Nov 21, 2014·The Plant Journal : for Cell and Molecular Biology·Alexandre BerrWen-Hui Shen
Mar 12, 2013·Genomics·Jeremy R ShearmanSithichoke Tangphatsornruang
Apr 12, 2014·Frontiers in Plant Science·Mara CucinottaIrma Roig-Villanova
Nov 26, 2010·Current Biology : CB·Xiaochun GeHong Ma
Jan 1, 2016·Biologie aujourd'hui·Alexandre BerrWen-Hui Shen
Dec 1, 2017·Frontiers in Plant Science·Jennifer C Fletcher
Feb 2, 2019·PLoS Genetics·Mathias NielsenSebastian Marquardt
Mar 15, 2019·Journal of Research on Adolescence : the Official Journal of the Society for Research on Adolescence·Carlos F AylwinAlejandro Lomniczi
Jan 27, 2018·Science China. Life Sciences·Lingling ChengQianwen Sun
Mar 7, 2020·Protein Science : a Publication of the Protein Society·Huiyan ZhouLi Huang
May 31, 2020·International Journal of Molecular Sciences·Helena G SilvaM Manuela R Costa

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Methods Mentioned

BETA
acetylation
Immuno
ChIP
PCR
scanning electron microscopy
transmission electron microscopy
light microscopy

Software Mentioned

AtGenexpress
Roche Probe Library Primer Design
Primer Design
limma
Vector NTI software ( Invitrogen )
LC480

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