The asparagine-transamidosome from Helicobacter pylori: a dual-kinetic mode in non-discriminating aspartyl-tRNA synthetase safeguards the genetic code.

Nucleic Acids Research
Frédéric FischerDaniel Kern

Abstract

Helicobacter pylori catalyzes Asn-tRNA(Asn) formation by use of the indirect pathway that involves charging of Asp onto tRNA(Asn) by a non-discriminating aspartyl-tRNA synthetase (ND-AspRS), followed by conversion of the mischarged Asp into Asn by the GatCAB amidotransferase. We show that the partners of asparaginylation assemble into a dynamic Asn-transamidosome, which uses a different strategy than the Gln-transamidosome to prevent the release of the mischarged aminoacyl-tRNA intermediate. The complex is described by gel-filtration, dynamic light scattering and kinetic measurements. Two strategies for asparaginylation are shown: (i) tRNA(Asn) binds GatCAB first, allowing aminoacylation and immediate transamidation once ND-AspRS joins the complex; (ii) tRNA(Asn) is bound by ND-AspRS which releases the Asp-tRNA(Asn) product much slower than the cognate Asp-tRNA(Asp); this kinetic peculiarity allows GatCAB to bind and transamidate Asp-tRNA(Asn) before its release by the ND-AspRS. These results are discussed in the context of the interrelation between the Asn and Gln-transamidosomes which use the same GatCAB in H. pylori, and shed light on a kinetic mechanism that ensures faithful codon reassignment for Asn.

References

Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·T H Fraser, A Rich
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M Sprinzl, F Cramer
Jan 1, 1989·Progress in Biophysics and Molecular Biology·A Danchin
Aug 1, 1972·The Biochemical Journal·R B Loftfield, D Vanderjagt
Sep 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·M Wilcox, M Nirenberg
Jan 15, 1994·European Journal of Biochemistry·M StanzelM Sprinzl
Aug 15, 1996·Nature·A W CurnowD Söll
Jan 1, 1996·Biochimie·R GiegéD Moras
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·A W CurnowD Söll
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·H D Becker, D Kern
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·A W CurnowD Söll
Sep 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Stephane SkouloubrisTamara L Hendrickson
Nov 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Juan C SalazarDieter Söll
Aug 10, 2005·The Journal of Biological Chemistry·Songon An, Karin Musier-Forsyth
Jul 18, 2006·Journal of Molecular Biology·Chun-Mei ZhangYa-Ming Hou
Nov 1, 2006·Nucleic Acids Research·Marc BaillyHubert Dominique Becker
Feb 7, 2008·Nucleic Acids Research·Kelly SheppardDieter Söll
Apr 22, 2008·Nature Structural & Molecular Biology·Chun-Mei ZhangYa-Ming Hou
Apr 29, 2008·Proceedings of the National Academy of Sciences of the United States of America·Claire PujolAnne-Marie Duchêne
Oct 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Benfang RuanDieter Söll
Apr 22, 2009·Annual Review of Microbiology·Jiqiang LingMichael Ibba
Sep 17, 2009·Nucleic Acids Research·Keng-Ming Chang, Tamara L Hendrickson
Nov 27, 2009·The Journal of Biological Chemistry·Min TanEn-Duo Wang
Apr 7, 2010·Infection and Immunity·Damien LeducHilde de Reuse
Nov 17, 2010·Nature Reviews. Microbiology·Noah M ReynoldsMichael Ibba
Jul 30, 2011·The Journal of Biological Chemistry·Mario H BarrosAlexander Tzagoloff

❮ Previous
Next ❯

Citations

Dec 22, 2012·The Journal of Biological Chemistry·Gayathri N SilvaTamara L Hendrickson
Jul 9, 2014·Proceedings of the National Academy of Sciences of the United States of America·Yuchen LiuDieter Söll
Jan 26, 2016·Journal of Molecular Biology·Nilendra NairKelly Sheppard
Dec 31, 2014·Proceedings of the National Academy of Sciences of the United States of America·Tateki SuzukiMin Yao
Jun 12, 2019·IUBMB Life·Catherine Florentz, Richard Giegé

❮ Previous
Next ❯

Methods Mentioned

BETA
aminoacylation
gel-filtration
size-exclusion chromatography
size chromatography
gel filtration
amidotransferases

Software Mentioned

BLAST

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.