The Aspergillus flavus Histone Acetyltransferase AflGcnE Regulates Morphogenesis, Aflatoxin Biosynthesis, and Pathogenicity

Frontiers in Microbiology
Huahui LanShihua Wang

Abstract

Histone acetyltransferases (HATs) help regulate fungal development and the production of secondary metabolites. In this study, we determined that the HAT AflGcnE influenced morphogenesis and aflatoxin biosynthesis in Aspergillus flavus. We observed that AflGcnE localized to the nucleus and cytoplasm during the conidial production and germination stages, while it was located mainly in the nucleus during the hyphal development stage. Deletion of AflgcnE inhibited the growth of A. flavus and decreased the hydrophobicity of the cell surface. The ΔAflgcnE mutant exhibited a lack of asexual sporulation and was unable to generate sclerotia. Additionally, AflgcnE was required to maintain cell wall integrity and genotoxic stress responses. Importantly, the ΔAflgcnE mutant did not produce aflatoxins, which was consistent with a significant down-regulation of aflatoxin gene expression levels. Furthermore, our data revealed that AflgcnE is a pathogenicity factor required for colonizing maize seeds. In summary, we revealed that A. flavus AflGcnE is crucial for morphological development, aflatoxin biosynthesis, stress responses, and pathogenicity. Our findings help clarify the functional divergence of GcnE orthologs, and may provide a possib...Continue Reading

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Citations

May 8, 2019·International Journal of Molecular Sciences·Saleem AhmadYu Wang
Jul 14, 2019·FEMS Microbiology Reviews·Jérôme Collemare, Michael F Seidl
Apr 16, 2019·Molecular Plant-microbe Interactions : MPMI·Huahui LanShihua Wang
Jun 6, 2018·Frontiers in Cellular and Infection Microbiology·Guang YangShihua Wang
Mar 15, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Natália Sayuri WassanoAndré Damasio
Feb 13, 2021·Journal of Fungi·Charmaine RetanalJennifer Geddes-McAlister
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Sep 6, 2020·Cell Chemical Biology·Tomohiro FurukawaShohei Sakuda
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Apr 21, 2021·Environmental Microbiology Reports·Fernando Uriel Rojas-Rojas, Julio C Vega-Arreguín
Apr 29, 2021·Annual Review of Phytopathology·Wende LiuXiao-Lin Chen

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Datasets Mentioned

BETA
AJS29493.1

Methods Mentioned

BETA
acetylation
gene knockout
PCR
reverse transcription PCR
immunoprecipitation assay
electrophoresis
acetylating

Software Mentioned

BLAST
MEGA
ClustalW
GraphPad Prism
DOG

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Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.