The Aspergillus fumigatus cspA gene encoding a repeat-rich cell wall protein is important for normal conidial cell wall architecture and interaction with host cells.

Eukaryotic Cell
Emma LevdanskyNir Osherov

Abstract

cspA (for cell surface protein A) encodes a repeat-rich glycophosphatidylinositol (GPI)-anchored cell wall protein (CWP) in the pathogenic fungus Aspergillus fumigatus. The number of repeats in cspA varies among isolates, and this trait is used for typing closely related strains of A. fumigatus. We have previously shown that deletion of cspA is associated with rapid conidial germination and reduced adhesion of dormant conidia. Here we show that cspA can be extracted with hydrofluoric acid (HF) from the cell wall, suggesting that it is a GPI-anchored CWP. The cspA-encoded CWP is unmasked during conidial germination and is surface expressed during hyphal growth. Deletion of cspA results in weakening of the conidial cell wall, whereas its overexpression increases conidial resistance to cell wall-degrading enzymes and inhibits conidial germination. Double mutant analysis indicates that cspA functionally interacts with the cell wall protein-encoding genes ECM33 and GEL2. Deletion of cspA together with ECM33 or GEL2 results in strongly reduced conidial adhesion, increased disorganization of the conidial cell wall, and exposure of the underlying layers of chitin and beta-glucan. This is correlated with increasing susceptibility of the...Continue Reading

References

Oct 1, 1994·Infection and Immunity·N ThauS Paris
Jun 13, 1998·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D W Denning
Mar 7, 2003·Applied and Environmental Microbiology·Sophie ParisJean Paul Latgé
Jul 8, 2003·Yeast·Piet W J De GrootFrans M Klis
Jun 16, 2004·Nature Reviews. Microbiology·Kevin J VerstrepenGerald R Fink
May 18, 2005·Fungal Genetics and Biology : FG & B·Piet W J De GrootFrans M Klis
Aug 9, 2005·Nature Genetics·Kevin J VerstrepenGerald R Fink
Feb 24, 2006·The Journal of Biological Chemistry·Margit EckerWidmar Tanner
Mar 7, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Geoffrey M GersukKieren A Marr
Mar 25, 2006·Molecular Microbiology·Kevin J Verstrepen, Frans M Klis
Mar 29, 2006·Trends in Genetics : TIG·Yechezkel Kashi, David G King
May 5, 2006·Applied and Environmental Microbiology·Sandrine ChabaneJean-Paul Latgé
Jun 15, 2007·Eukaryotic Cell·Emma LevdanskyNir Osherov
Jul 13, 2007·Fungal Genetics and Biology : FG & B·Xiaomin ZhaoLois L Hoyer
Feb 6, 2008·Microbes and Infection·Kathrin LutherFrank Ebel
Apr 1, 2008·Journal of Microbiological Methods·S Arunmozhi BalajeeCorné H W Klaassen
Feb 18, 2009·Molecular Microbiology·Daniele E EjzykowiczScott G Filler
May 1, 2009·The New England Journal of Medicine·Brahm H Segal
Jul 15, 2009·Clinical Microbiology Reviews·Taylor R T Dagenais, Nancy P Keller
Aug 14, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Z ErjavecP E Verweij
Aug 29, 2009·Nature·Vishukumar AimaniandaJean-Paul Latgé

❮ Previous
Next ❯

Citations

Feb 12, 2013·Eukaryotic Cell·Piet W J de GrootNeeraj Chauhan
Aug 25, 2011·Future Microbiology·Frans M KlisStanley Brul
Aug 22, 2013·Medical Mycology·Savneet Kaur, Shweta Singh
Jul 10, 2012·Biochimica Et Biophysica Acta·Carl H MesarichAndrew J Dingley
Apr 20, 2016·Frontiers in Microbiology·Carys A CroftScott J Tebbutt
Mar 31, 2015·BioMed Research International·Zhongqi FanHuimei Yu
Jul 26, 2011·Current Opinion in Microbiology·Donald C Sheppard
Mar 2, 2013·Studies in Mycology·R BleichrodtH A B Wösten
Nov 19, 2011·FEMS Microbiology Letters·Gordon RamageCraig Williams
Jun 4, 2016·Journal of Basic Microbiology·Almudena Aranda-MartinezLuis Vicente Lopez-Llorca
Sep 1, 2015·PLoS Neglected Tropical Diseases·Clarissa Xavier Resende ValimMaria Célia Jamur
Feb 13, 2018·Archives of Microbiology·Suhas Ballal, Shashikala R Inamdar
Jan 27, 2018·Journal of Fungi·Margherita BertuzziElaine M Bignell
Nov 20, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Susann HartungMarie von Lilienfeld-Toal
Sep 22, 2018·Medical Mycology·Sara GagoPaul Bowyer
Sep 13, 2019·PLoS Pathogens·Matthew G BlangoAxel A Brakhage
Jan 1, 2020·Current Protein & Peptide Science·Laura C García-CarneroAlma K Tamez-Castrellón
Dec 9, 2017·Database : the Journal of Biological Databases and Curation·Matheus Eloy FrancoAna Lúcia Fachin
Jan 18, 2019·International Journal of Molecular Sciences·Federico Lopez-MoyaLuis Vicente Lopez-Llorca
Jul 31, 2019·Applied Microbiology and Biotechnology·Jesús Urbar-UlloaJorge Verdín
Jan 15, 2021·Comprehensive Reviews in Food Science and Food Safety·Razieh RajestaryGianfranco Romanazzi
Sep 10, 2015·Microbiology Spectrum·Anne Beauvais, Jean-Paul Latgé
Apr 3, 2020·Journal of Proteome Research·Matthew G BlangoAxel A Brakhage

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.