The Aspergillus nidulans GATA transcription factor gene areB encodes at least three proteins and features three classes of mutation

Molecular Microbiology
H ConlonM X Caddick

Abstract

In Aspergillus nidulans, the principal transcription factor regulating nitrogen metabolism, AREA, belongs to the GATA family of DNA-binding proteins. In seeking additional GATA factors, we have cloned areB, which was originally identified via a genetic screen for suppressors of areA loss-of-function mutations. Based on our analysis, areB is predicted to encode at least three distinct protein products. These arise from the use of two promoters, differential splicing and translation initiating at AUG and non-AUG start codons. All the putative products include a GATA domain and a putative Leu zipper. These regions show strong sequence similarity to regulatory proteins from Saccharomyces cerevisiae (Dal80p and Gzf3p), Penicillium chrysogenum (NREB) and Neurospora crassa (ASD4). We have characterized three classes of mutation in areB; the first are loss-of-function mutations that terminate the polypeptides within or before the GATA domain. The second class truncates the GATA factor either within or upstream of the putative Leu zipper but retains the GATA domain. The third class fuses novel gene sequences to areB with the potential to produce putative chimeric polypeptides. These novel gene fusions transform the putative negative-act...Continue Reading

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Citations

Sep 12, 2006·Eukaryotic Cell·Igor Y MorozovHerbert N Arst
Mar 22, 2014·BMC Genomics·Richard B ToddRonald P de Vries
Feb 12, 2013·The Journal of Biological Chemistry·Jiajie WeiMatthew S Sachs
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Sep 1, 2006·Medical Mycology·Mark X CaddickMeriel G Jones
Apr 4, 2007·The Journal of Biological Chemistry·Laura Murcia-FloresRosa M Ruiz-Vázquez
Jun 16, 2017·Biotechnology for Biofuels·Tiziano BenocciRonald P de Vries
Jan 19, 2020·Applied and Environmental Microbiology·Wei LuoXiaobin Yu
Jul 22, 2020·Molecular Microbiology·Kristina ShostakRajagopal Subramaniam
Jun 26, 2020·Microbial Pathogenesis·Vanessa Rafaela Milhomem Cruz-LeiteClayton Luiz Borges

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