The assembly of the measles virus nucleoprotein into nucleocapsid-like particles is modulated by the phosphoprotein

Virology
D SpehnerP M Howley

Abstract

Measles virus nucleoprotein encoded from the vaccinia virus genome assembles into nucleocapsids similar in many respects to those observed during a natural measles virus infection. The influence of the measles virus phosphoprotein on nucleocapsid assembly has been studied using a vaccinia virus recombinant encoding both the nucleoprotein and the phosphoprotein. Infection of cells with the virus recombinant resulted in the formation of cytoplasmic inclusions in which the nucleoprotein and the phosphoprotein colocalized. Electron microscopic examination suggested that these inclusions contained characteristic nucleocapsid filaments. The buoyant density of nucleocapsids assembled in the presence of the phosphoprotein was found to be slightly higher than that of nucleocapsids assembled in its absence. Furthermore, the phosphoprotein partially inhibited the formation of nucleocapsids, a process which was extremely efficient when the nucleoprotein was expressed alone. Analysis of the nucleic acid content of nucleocapsids showed that they packaged heterologous RNA into a micrococcal nuclease-resistant form. These experiments demonstrate that the measles virus phosphoprotein regulates the efficiency with which the nucleoprotein assembl...Continue Reading

References

Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S Munro, T Maniatis

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Citations

Oct 12, 2000·Journal of Virology·S VincentS N Manié
Apr 23, 2002·Journal of Virology·Richard K PlemperRoberto Cattaneo
Mar 22, 2002·Journal of Virology·Benjamin R tenOeverJohn Hiscott
Jul 29, 2004·Journal of Virology·Richard L KingstonLeslie S Gay
May 14, 2005·Journal of Virology·Sébastien PlumetDenis Gerlier
Jun 11, 2014·Acta Crystallographica. Section D, Biological Crystallography·David BlocquelSonia Longhi
Jan 9, 2004·Journal of Biotechnology·Rimantas SlibinskasKestutis Sasnauskas
Feb 1, 2005·Virus Research·Mindaugas JuozapaitisKestutis Sasnauskas
Jul 19, 2002·The Journal of General Virology·David BhellaRobert P Yeo
Feb 22, 2013·The Journal of General Virology·Jana Baron, Michael Baron
Oct 29, 1998·Virology·S BeckerE Mühlberger
Jul 31, 2013·Virus Research·Andressa P OliveiraArmando M Ventura
Aug 2, 2011·Molecular BioSystems·Johnny Habchi, Sonia Longhi
Nov 8, 2006·Nature Reviews. Microbiology·William J Moss, Diane E Griffin
Aug 28, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shin-ichi YokotaNobuhiro Fujii
Nov 13, 2019·Virus Research·Bruno Rafael Pereira LopesKarina Alves Toledo
Jun 17, 2006·Science·Aurélie A V AlbertiniRob W H Ruigrok

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