The association between Salt-inducible kinase 2 (SIK2) and gamma isoform of the regulatory subunit B55 of PP2A (B55gamma) contributes to the survival of glioma cells under glucose depletion through inhibiting the phosphorylation of S6K

Cancer Cell International
Ya-Nan LiJian-Min Liu

Abstract

PPP2R2C encodes a gamma isoform of the regulatory subunit B55 subfamily consisting PP2A heterotrimeric with A and C subunits. Currently, the precise functions of B55gamma in cancer are still under investigating. In this project, we reported a novel function of B55gamma in the regulation of glucose metabolism in Glioma cells. Western blot and immunoprecipitation were performed to determine protein expression and interaction. Cell viability was measured by Typan Blue staining and direct cell counting using hematocytometer. siRNA technology was used to down regulate protein expression. Glucose uptake and lactate product were suppressed by overexpression of B55gamma in Glioma cells. In addition, cancer cells with larger amount of B55gamma showed higher survival advantages in response to glucose starvation through the dephosphorylation of S6K. From proteomic analysis, we found B55gamma binds with and up regulates SIK2 through the stabilization of SIK2 protein which is required for the B55gamma-mediated suppression of S6K pathway. Knocking down of SIK2 in B55gamma over expressing cells recovered the phosphorylation of S6K. In summary, our project will provide novel insight into the design and development of therapeutic strategies to ...Continue Reading

References

May 10, 2000·The Journal of Biological Chemistry·D AbrahamM Baccarini
Dec 25, 2003·Trends in Endocrinology and Metabolism : TEM·Mitsuhiro OkamotoYoshiko Katoh
Oct 24, 2006·Nature Reviews. Molecular Cell Biology·Nicholas K Tonks
Feb 9, 2007·The Journal of Biological Chemistry·Catherine E GleasonMorris J Birnbaum
May 23, 2009·Science·Matthew G Vander HeidenCraig B Thompson
May 26, 2009·Biochemical and Biophysical Research Communications·Dongsoon Bae, Susan Ceryak
Oct 29, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Chi V DangPing Gao
Jan 12, 2010·Current Opinion in Cell Biology·Eric C Cheung, Karen H Vousden
Jul 6, 2010·European Journal of Medical Genetics·Liesbeth BackxHilde Van Esch
Oct 1, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Y DuT S Kern
Dec 17, 2010·Cancer Research·Anna A SablinaWilliam C Hahn
Jan 12, 2011·Neuron·Tsutomu SasakiKazuo Kitagawa
Aug 19, 2011·Molecular Systems Biology·Daniela GaglioFerdinando Chiaradonna
Mar 5, 2013·Cancer Letters·Parthasarathy SeshacharyuluSurinder K Batra
Mar 16, 2013·Molecular Cancer Research : MCR·Eric G BluemnPeter S Nelson
Oct 5, 2013·Experimental & Molecular Medicine·Miran JangJinhwa Lee

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Methods Mentioned

BETA
electrophoreses
transfection
immunoprecipitation

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