PMID: 5513607May 1, 1970

The association of a class of saltatory movements with microtubules in cultured cells

The Journal of Cell Biology
J J FREED, M M Lebowitz

Abstract

Particulate structures in the cytoplasm of HeLa and other cultured cells in interphase undergo rapid individual linear displacements (long saltatory movements, LSM). By the use of time-lapse microscopy to locate saltating particles prior to fixation and histochemical examination of the cells, structures of several kinds have been shown to move in this manner. Elements that show LSM include lysosomes, pinosomes, ingested carbon particles, lipoidal granules, and unidentified particles that appear as bright objects in positive phase contrast. The pattern of movement of the particles suggests the presence of linear guiding elements radially disposed from the cytocenter (centriole region). The participation of microtubules in these movements is inferred from the observation that LSM cease after treatment with drugs which depolymerize microtubules, i.e., colchicine, Vinblastine, and podophyllin. The directions of the microtubules in the cytoplasm of HeLa cells found by electron microscopy are consistent with the aster-like configuration predicted from study of LSM. Further support for this arrangement of cytoplasmic microtubules is provided by light microscope observations of colchicine-sensitive radial arrays of acid phosphatase gra...Continue Reading

References

Nov 1, 1975·Arthritis and Rheumatism·S E Malawista
Nov 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J A Swanson
Jul 1, 1990·Journal of Electron Microscopy Technique·D N FurnessP S Steyger
Mar 29, 1973·Zeitschrift für Zellforschung und mikroskopische Anatomie·D J Wigglesworth, A F Hayward
Aug 14, 1973·Zeitschrift für Zellforschung und mikroskopische Anatomie·G E Tyson, R E Bulger
Mar 30, 1976·Molecular & General Genetics : MGG·K J Aufderheide, R G Johnson
Jan 1, 1970·Protoplasma·E J Sanders, S E Zalik
Jan 1, 1985·The Journal of Membrane Biology·W S May, P Cuatrecasas
Sep 1, 1978·Acta diabetologica latina·R B PuriV K Mohan Rao
Apr 9, 2004·European Biophysics Journal : EBJ·D B HillG Holzwarth
Apr 16, 1971·Brain Research·F E SamsonS L Twomey
Nov 1, 1971·Developmental Biology·B Burnside
Feb 1, 1986·Developmental Biology·T P FlemingS J Pickering
Jan 1, 1979·Experimental Cell Research·W W FrankeM Osborn
Jan 1, 1977·Tissue & Cell·J S Hyams, H Stebbings
Jan 1, 1974·Progress in Biophysics and Molecular Biology·K Roberts
May 1, 1982·Cell·K R Porter, M A McNiven
Feb 1, 1989·Trends in Neurosciences·R B ValleeB M Paschal
Apr 23, 2003·Experimental Cell Research·Ralph W KunclRobert Adams
Jun 1, 1972·Journal of Ultrastructure Research·D BrandesS Barnard
Dec 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·J A Williams, J Wolff
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·R B ZurierG Weissmann
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·O Y IvanovaI M Gelfand
Aug 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J W ShayD M Prescott
Mar 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·M Osborn, K Weber
Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·F R Frankel
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J De MeyG Geuens

Citations

Dec 1, 1968·The Journal of Cell Biology·K V Holmes, P W Choppin
Oct 1, 1967·The Journal of Cell Biology·B R BrinkleyL C Richardson
Feb 1, 1968·The Journal of Cell Biology·S N Klaus
Feb 1, 1969·The Journal of Cell Biology·J L Rosenbaum, K Carlson
Sep 1, 1968·The Journal of Cell Biology·H IshikawaH Holtzer
Jul 1, 1967·Journal of Ultrastructure Research·O Behnke, T Zelander
Aug 1, 1967·The Journal of Cell Biology·G G Borisy, E W Taylor
Sep 1, 1964·Experimental Cell Research·T R MUNROJ T DINGLE
Sep 1, 1964·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E ROBBINS, N K GONATAS
Oct 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·B BYERS, K R Porter
Dec 1, 1964·The Anatomical Record·M D MASER, C W PHILPOTT

Related Concepts

Acid Phosphatase
Vinblastine
Interphase
Protoplasm
Aster Microtubules
Sample Fixation
Aster Plant
Culture Techniques
Microtubules
Electron Microscopy, Diagnostic

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