The association of GPR85 with PSD-95-neuroligin complex and autism spectrum disorder: a molecular analysis

Molecular Autism
Eriko Fujita-JimboT Momoi

Abstract

Autism spectrum disorder (ASD) has a complex genetic etiology. Some symptoms and mutated genes, including neuroligin (NLGN), neurexin (NRXN), and SH3 and multiple ankyrin repeat domains protein (SHANK), are shared by schizophrenia and ASD. Little is known about the molecular pathogenesis of ASD. One of the possible molecular pathogenesis is an imbalance of excitatory and inhibitory receptors linked with the NLGN-PSD-95-SHANK complex via postsynaptic density protein/Drosophila disc large tumor suppressor/zonula occludens-1 protein (PDZ) binding. In the present study, we focused on GPR85 as a candidate gene for ASD because the C-terminal amino acid sequence of GPR85 [Thr-Cys-Val-Ile (YCVI)] is classified as a type II PDZ-binding motif, and GPR85 is a risk factor for schizophrenia. GPR85 is an orphan receptor that regulates neural and synaptic plasticity and modulates diverse behaviors, including learning and memory. While searching for molecules that associate with GPR85, we found that GPR85 was associated with postsynaptic density protein (PSD)-95 linked with NLGN in the brain. We examined the proteins that associate with the C-terminal sequence of GPR85 by pull-down assay and immunoblot analysis and searched for a mutation of t...Continue Reading

References

Sep 5, 1997·Science·M IrieT C Südhof
Jul 2, 1998·Biochemical and Biophysical Research Communications·Y KourokuT Momoi
Jun 2, 2000·Biochemical and Biophysical Research Communications·M MatsumotoK Furuichi
Apr 2, 2003·Nature Genetics·Stéphane JamainUNKNOWN Paris Autism Research International Sibpair Study
Apr 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Demetrios K VassilatisGeorge A Gaitanaris
Jun 22, 2005·Brain & Development·Hong LiMariko Y Momoi
Jun 24, 2006·Cellular and Molecular Life Sciences : CMLS·M-F Lisé, A El-Husseini
Aug 10, 2006·Journal of Autism and Developmental Disorders·Lonnie ZwaigenbaumMarian Sigman
Jan 9, 2008·American Journal of Human Genetics·Betul BakkalogluMatthew W State
Apr 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Mitsuyuki MatsumotoDaniel R Weinberger
Sep 9, 2008·Child and Adolescent Psychiatric Clinics of North America·David J PoseyChristopher J McDougle
Oct 30, 2008·Biochemical and Biophysical Research Communications·Yu ZhilingMariko Y Momoi
Apr 14, 2010·Proceedings of the National Academy of Sciences of the United States of America·Julie GauthierUNKNOWN S2D Team
Jul 24, 2013·Annual Review of Genomics and Human Genetics·Guillaume HuguetThomas Bourgeron
Jan 28, 2014·Nature·Menachem FromerMichael C O'Donovan

❮ Previous
Next ❯

Citations

Sep 16, 2016·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Dana HollenbeckFady M Mikhail
May 16, 2018·Autism Research : Official Journal of the International Society for Autism Research·Daoyin DongPeixin Yang
Sep 25, 2017·Psychiatry and Clinical Neurosciences·Mary M Y Waye, Ho Yu Cheng
Jul 8, 2016·The Journal of Pharmacology and Experimental Therapeutics·Yutaka Nakagawa, Kenji Chiba
Nov 25, 2017·Progress in Neuro-psychopharmacology & Biological Psychiatry·Austin A Coley, Wen-Jun Gao
Jun 10, 2021·Scientific Reports·Timothy S BretonMatthew A DiMaggio

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transfection
pull-down

Software Mentioned

SIFT
Image J
Mutation Taster

Related Concepts

Related Feeds

Autism

Autism spectrum disorder is associated with challenges with social skills, repetitive behaviors, and often accompanied by sensory sensitivities and medical issues. Here is the latest research on autism.

Autism: Treatment Targets

The absence of effective treatments for autism are due to the high clinical and genetic heterogeneity between affected individuals, restricted knowledge of the underlying pathophysiological mechanisms, and the lack of reliable diagnostic biomarkers. Identification of more homogenous biological subgroups is therefore essential for the development of novel treatments based on the molecular mechanisms underpinning autism and autism spectrum disorders. Find the latest research on autism treatment targets here.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.