The association of H-2Ld with human beta-2 microglobulin induces localized conformational changes in the alpha-1 and -2 superdomain.

Immunogenetics
M C NietoR S Goodenow

Abstract

We have analyzed changes in the antigenicity of major histocompatibility complex class I molecules resulting from the association of human beta-2 micro-globulin (B2m) with the mouse class I heavy chain. In particular, the H-2Ld molecule exhibited enhanced cross-reactivity for the 34-1-2 monoclonal antibody. In order to assess the nature of this structural alteration induced by human B2m, we utilized H-2 class I hybrid molecules in the mapping of the 34-1-2 determinant to the helical region of the alpha-1 domain. H-2Ld class I hybrid molecules were then used to establish the importance of the alpha-2 and -3 domains in the observed increase of 34-1-2 cross-reactivity following exchange with human B2m. The H-2Ld hybrids suggest that alterations in interdomain contact are responsible for enhanced 34-1-2 cross-reactivity on the H-2Ld molecule. It is likely that this alteration arises through changes in class I conformation at regions of the molecule distant from points of contact between B2m and the class I molecule. This suggests that perturbations induced by association of human B2m with H-2Ld can affect the conformation of the alpha-1 and -2 superdomain. That class I antigenic determinants are altered by the association of human ...Continue Reading

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Citations

Oct 30, 2008·Immunogenetics·Laura C SimoneJoyce C Solheim
Aug 19, 2009·Immunology and Cell Biology·Laura C SimoneJoyce C Solheim
Nov 1, 1994·Developmental and Comparative Immunology·J C KunichM C Nieto
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Jul 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amit TuliJoyce C Solheim
Jun 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·J MichaëlssonK Kärre
Feb 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Heth R TurnquistJoyce C Solheim
Jul 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Loralyn A Benoît, Rusung Tan
Sep 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Loralyn A Benoit, Rusung Tan

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