The association of PI3 kinase signaling and chemoresistance in advanced ovarian cancer.

Molecular Cancer Therapeutics
Craig P CardenUdai Banerji

Abstract

Evidence that the phosphoinositide 3-kinase (PI3K) pathway is deregulated in ovarian cancer is largely based on the analysis of surgical specimens sampled at diagnosis and may not reflect the biology of advanced ovarian cancer. We aimed to investigate PI3K signaling in cancer cells isolated from patients with advanced ovarian cancer. Ascites samples were analyzed from 88 patients, of whom 61 received further treatment. Cancer cells were immunomagnetically separated from ascites, and the signaling output of the PI3K pathway was studied by quantifying p-AKT, p-p70S6K, and p-GSK3β by ELISA. Relevant oncogenes, such as PIK3CA and AKT, were sequenced by PCR-amplified mass spectroscopy detection methods. In addition, PIK3CA and AKT2 amplifications and PTEN deletions were analyzed by FISH. p-p70S6K levels were significantly higher in cells from 37 of 61 patients who did not respond to subsequent chemotherapy (0.7184 vs. 0.3496; P = 0.0100), and this difference was greater in patients who had not received previous chemotherapy. PIK3CA and AKT mutations were present in 5% and 0% of samples, respectively. Amplification of PIK3CA and AKT2 and deletion of PTEN was seen in 10%, 10%, and 27% of samples, respectively. Mutations of PIK3CA and ...Continue Reading

References

Mar 22, 2002·The Lancet Oncology·D M Parkin
Nov 3, 2004·Cancer Research·Ian G CampbellWayne A Phillips
Feb 16, 2005·Human Mutation·Yun WangAnne-Lise Børresen-Dale
Apr 20, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Douglas A LevineJeff Boyd
Jun 17, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Amanda F BakerGarth Powis
Oct 6, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Karine Passebosc-FaureChristian Genin
May 25, 2006·Cancer Biology & Therapy·Kentaro NakayamaIe-Ming Shih
Jan 30, 2007·Human Pathology·Julia WillnerElizabeth M Swisher
Mar 14, 2007·International Journal of Cancer. Journal International Du Cancer·Kentaro NakayamaTian-Li Wang
Mar 23, 2007·Virchows Archiv : an International Journal of Pathology·Joachim WoenckhausIrina Fenic
Jul 20, 2007·Oncogene·G AttardUNKNOWN Transatlantic Prostate Group
May 28, 2008·Oncogene·F E BleekerA Bardelli
Nov 26, 2008·Cancer Biology & Therapy·Iwona K KolasaJolanta Kupryjanczyk
Feb 25, 2009·Nature Reviews. Cancer·Timothy A YapStan B Kaye
Jul 30, 2009·Molecular Cancer·Jehad AbubakerKhawla S Al-Kuraya
Jan 28, 2010·British Journal of Cancer·A H M ReidUNKNOWN Transatlantic Prostate Group
Mar 11, 2010·Biochemical and Biophysical Research Communications·Dong-Jun PengGen Sheng Wu
May 11, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Carol O'BrienMark R Lackner
Jul 6, 2010·Biochemical and Biophysical Research Communications·Chuan-Xiu BianBing-Hua Jiang
Jan 11, 2011·Molecular Cancer Therapeutics·Filip JankuRazelle Kurzrock
Feb 22, 2011·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Dana FaratianDavid J Harrison
Mar 15, 2011·Cancer Cell·Jean-Philippe TheurillatWilhelm Krek
Jul 12, 2011·The Lancet Oncology·Susanna L Cooke, James D Brenton
Dec 14, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Paul A Clarke, Paul Workman
Dec 20, 2011·Cancer Discovery·Chad V PecotAnil K Sood

❮ Previous
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Citations

Dec 7, 2013·Annals of Oncology : Official Journal of the European Society for Medical Oncology·J LopezS B Kaye
Sep 17, 2013·International Journal of Molecular Sciences·Eleftherios P SamartzisPatrick Imesch
Dec 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Vanessa M PetersonRalph Weissleder
Mar 5, 2014·Carcinogenesis·Xiaobing DengEileen Friedman
Oct 10, 2013·Journal of Cellular Physiology·Sabita N Saldanha, Trygve O Tollefsbol
Sep 17, 2015·Endocrine-related Cancer·Helen GharwanChristina M Annunziata
Sep 14, 2016·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Patricia Mucci LoRusso
Apr 18, 2013·International Journal of Molecular Sciences·Zachary C Dobbin, Charles N Landen
Jan 24, 2019·Journal of Cellular and Molecular Medicine·Xuan JiangZhenyu Zhang
Feb 22, 2013·Nature Reviews. Cancer·Emma KippsStan B Kaye
Jul 15, 2015·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Rania M BakkarRussell R Broaddus
Jul 17, 2019·Biomarkers in Cancer·Ruchika PokhriyalGururao Hariprasad
Jun 22, 2017·The Journal of Biological Chemistry·Angela M GocherArthur M Edelman
Nov 3, 2020·Journal of Cell Communication and Signaling·Mohd Altaf NajarT S Keshava Prasad
Apr 15, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Pedro J BeltranGottfried E Konecny
Sep 19, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sarah BlagdenUdai Banerji
Jan 12, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Susana Banerjee, Stanley B Kaye
Mar 7, 2021·International Journal of Molecular Sciences·Abdelrahman M ElsayedCristian Rodriguez-Aguayo
Mar 18, 2021·Scientific Reports·Arnon Møldrup KnudsenBjarne Winther Kristensen

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