The ATC1 gene encodes a cell wall-linked acid trehalase required for growth on trehalose in Candida albicans.

The Journal of Biological Chemistry
Yolanda PedreñoEulogio Valentín

Abstract

After screening a Candida albicans genome data base, the product of an open reading frame (IPF 19760/CA2574) with 41% identity to Saccharomyces cerevisiae vacuolar acid trehalase (Ath1p) was identified and named Atc1p. The deduced amino acid sequence shows that Atc1p contains an N-terminal hydrophobic signal peptide and 20 potential sites for N-glycosylation. C. albicans homozygous mutants that lack acid trehalase activity were constructed by gene disruption at the two ATC chromosomal alleles. Analysis of these null mutants shows that Atc1p is localized in the cell wall and is required for growth on trehalose as a carbon source. An Atc1p endowed with acid trehalase activity was obtained by an in vtro transcription-translation coupled system. These results strongly suggest that ATC1 is the structural gene encoding cell wall acid trehalase in C. albicans. Determinations of ATC1 mRNA expression as well as acid trehalase activity in the presence and absence of glucose point out that ATC1 gene is regulated by glucose repression.

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Citations

Sep 6, 2008·Microbiology and Molecular Biology Reviews : MMBR·W LaJean Chaffin
May 17, 2011·Biochimica Et Biophysica Acta·Pilar González-PárragaJuan-Carlos Argüelles
Feb 24, 2009·International Journal of Medical Microbiology : IJMM·María Martínez-EsparzaJuan-Carlos Argüelles
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May 3, 2016·Biochimica Et Biophysica Acta·Sergi MaicasJuan-Carlos Argüelles
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Jan 12, 2020·Applied Microbiology and Biotechnology·Masayoshi Sakaguchi
Jul 18, 2020·International Journal of Microbiology·Napasawan PlabutongArsa Thammahong
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Apr 2, 2009·Biochemical and Biophysical Research Communications·Ruth Sánchez-FresnedaJuan-Carlos Argüelles

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