The ATC1 gene encodes a cell wall-linked acid trehalase required for growth on trehalose in Candida albicans.
Abstract
After screening a Candida albicans genome data base, the product of an open reading frame (IPF 19760/CA2574) with 41% identity to Saccharomyces cerevisiae vacuolar acid trehalase (Ath1p) was identified and named Atc1p. The deduced amino acid sequence shows that Atc1p contains an N-terminal hydrophobic signal peptide and 20 potential sites for N-glycosylation. C. albicans homozygous mutants that lack acid trehalase activity were constructed by gene disruption at the two ATC chromosomal alleles. Analysis of these null mutants shows that Atc1p is localized in the cell wall and is required for growth on trehalose as a carbon source. An Atc1p endowed with acid trehalase activity was obtained by an in vtro transcription-translation coupled system. These results strongly suggest that ATC1 is the structural gene encoding cell wall acid trehalase in C. albicans. Determinations of ATC1 mRNA expression as well as acid trehalase activity in the presence and absence of glucose point out that ATC1 gene is regulated by glucose repression.
References
Identification of wall-specific antigens synthesized during germ tube formation by Candida albicans.
Trehalose metabolism in Escherichia coli: stress protection and stress regulation of gene expression
Citations
Pga26 mediates filamentation and biofilm formation and is required for virulence in Candida albicans
The involvement of the Candida glabrata trehalase enzymes in stress resistance and gut colonization.
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