The atomic resolution crystal structure of atratoxin determined by single wavelength anomalous diffraction phasing.

The Journal of Biological Chemistry
Xiaohua LouQuan Hao

Abstract

By using single wavelength anomalous diffraction phasing based on the anomalous signal from copper atoms, the crystal structure of atratoxin was determined at the resolution of 1.5 A and was refined to an ultrahigh resolution of 0.87 A. The ultrahigh resolution electron density maps allowed the modeling of 38 amino acid residues in alternate conformations and the location of 322 of 870 possible hydrogen atoms. To get accurate information at the atomic level, atratoxin-b (an analog of atratoxin with reduced toxicity) was also refined to an atomic resolution of 0.92 A. By the sequence and structural comparison of these two atratoxins, Arg(33) and Arg(36) were identified to be critical to their varied toxicity. The effect of copper ions on the distribution of hydrogen atoms in atratoxin was discussed, and the interactions between copper ions and protein residues were analyzed based on a statistical method, revealing a novel pentahedral copper-binding motif.

References

Jun 13, 1979·Biochemical and Biophysical Research Communications·M R KimballB W Low
Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·B W LowJ S Richardson
Aug 1, 1992·Acta Crystallographica. Section B, Structural Science·P SaludjianA Ducruix
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
May 1, 1988·Acta Crystallographica. Section A, Foundations of Crystallography·J L SmithB W Low
Jan 1, 1985·Toxicon : Official Journal of the International Society on Toxinology·S D Aird, I I Kaiser
Dec 16, 1985·European Journal of Biochemistry·P E BourneB W Low
Sep 21, 1984·Science·J P ChangeuxP Chemouilli
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M D WalkinshawA Maelicke
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·C VitaA Ménez
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·V NastopoulosD Tsernoglou
May 18, 1999·Acta Crystallographica. Section D, Biological Crystallography·E A Merritt
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Carole Fruchart-GaillardDenis Servent
May 15, 2002·The Journal of Biological Chemistry·Fatima Teixeira-ClercPascal Kessler
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Jan 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·M M Woolfson, J X Yao
Jul 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·A PerrakisV S Lamzin
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

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Sep 26, 2013·Advanced Drug Delivery Reviews·Yu ChengMaciej S Lesniak
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May 20, 2008·The FEBS Journal·Andrzej GalatAndré Ménez
Nov 7, 2016·Current Opinion in Structural Biology·Natalia G Esipova, Vladimir G Tumanyan
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