The ATP-sensitive K(+)-channel (K(ATP)) controls early left-right patterning in Xenopus and chick embryos.

Developmental Biology
Sherry AwMichael Levin

Abstract

Consistent left-right asymmetry requires specific ion currents. We characterize a novel laterality determinant in Xenopus laevis: the ATP-sensitive K(+)-channel (K(ATP)). Expression of specific dominant-negative mutants of the Xenopus Kir6.1 pore subunit of the K(ATP) channel induced randomization of asymmetric organ positioning. Spatio-temporally controlled loss-of-function experiments revealed that the K(ATP) channel functions asymmetrically in LR patterning during very early cleavage stages, and also symmetrically during the early blastula stages, a period when heretofore largely unknown events transmit LR patterning cues. Blocking K(ATP) channel activity randomizes the expression of the left-sided transcription of Nodal. Immunofluorescence analysis revealed that XKir6.1 is localized to basal membranes on the blastocoel roof and cell-cell junctions. A tight junction integrity assay showed that K(ATP) channels are required for proper tight junction function in early Xenopus embryos. We also present evidence that this function may be conserved to the chick, as inhibition of K(ATP) in the primitive streak of chick embryos randomizes the expression of the left-sided gene Sonic hedgehog. We propose a model by which K(ATP) channel...Continue Reading

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Citations

May 13, 2014·Reproductive Toxicology·Sonia Hernández-Díaz, Michael Levin
Aug 10, 2011·PloS One·Laura N VandenbergMichael Levin
Jul 28, 2011·Evolution; International Journal of Organic Evolution·Hiroki UtsunoEdmund Gittenberger
Feb 11, 2014·Biophysical Journal·Jennifer H HouAdam E Cohen
Feb 8, 2014·Communicative & Integrative Biology·Laura N VandenbergMichael Levin
Sep 17, 2016·Progress in Biophysics and Molecular Biology·Zhimin Li, Charles Shang
Feb 13, 2016·Scientific Reports·Chunmei LiDavid L Kaplan
Mar 4, 2014·Genesis : the Journal of Genetics and Development·Melanie TinglerMartin Blum
Mar 29, 2014·Genesis : the Journal of Genetics and Development·Michelle M Collins, Aimee K Ryan
Jun 11, 2017·Cold Spring Harbor Perspectives in Biology·Joseph ZinskiMary C Mullins
Oct 30, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Laura N Vandenberg, Michael Levin
Mar 10, 2018·Nature Communications·Vaibhav P PaiMichael Levin
Nov 17, 2011·Genesis : the Journal of Genetics and Development·Laura N Vandenberg, Michael Levin
Mar 20, 2015·Birth Defects Research. Part C, Embryo Today : Reviews·Katia CarneiroMaria I D Rossi
Jun 13, 2020·Frontiers in Cellular Neuroscience·Vaibhav P PaiMichael Levin
Apr 8, 2018·Progress in Biophysics and Molecular Biology·Alexis Pietak, Michael Levin

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