PMID: 9440534Jan 24, 1998Paper

The ATP synthase atpHAGDC (F1) operon from Rhodobacter capsulatus

Journal of Bacteriology
R BorgheseB A Melandri

Abstract

The atpHAGDC operon of Rhodobacter capsulatus, containing the five genes coding for the F1 sector of the ATP synthase, has been cloned and sequenced. The promoter region has been defined by primer extension analysis. It was not possible to obtain viable cells carrying atp deletions in the R. capsulatus chromosome, indicating that genes coding for ATP synthase are essential, at least under the growth conditions tested. We were able to circumvent this problem by combining gene transfer agent transduction with conjugation. This method represents an easy way to construct strains carrying mutations in indispensable genes.

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Citations

Jul 21, 2000·DNA Sequence : the Journal of DNA Sequencing and Mapping·T ExternbrinkP Dürre
Jun 7, 2006·Photosynthesis Research·Giorgio FortiGiuseppe Zucchelli
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·S C BakerR J van Spanning
Jun 13, 2006·Biochimica Et Biophysica Acta·Paola TurinaB Andrea Melandri

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