The ATPase ATP6V1A facilitates rabies virus replication by promoting virion uncoating and interacting with the viral matrix protein.

The Journal of Biological Chemistry
Xing LiuZhigao Bu

Abstract

Rabies virus (RABV) matrix protein (M) plays crucial roles in viral transcription, replication, assembly, and budding; however, its function during the early stage of virus replication remains unknown. Here, we mapped the protein interactome between RABV M and human host factors using a proteomic approach, finding a link to the V-type proton ATPase catalytic subunit A (ATP6V1A), which is located in the endosomes where RABV first enters. By downregulating or upregulating ATP6V1A expression in HEK293T cells, we found that ATP6V1A facilitated RABV replication. We further found that ATP6V1A was involved in the dissociation of incoming viral M proteins during viral uncoating. Coimmunoprecipitation demonstrated that M interacted with the full length or middle domain of ATP6V1A, which was dependent on the lysine residue at position 256 and the glutamic acid residue at position 279. RABV growth and uncoating in ATP6V1A-depleted cells was restored by trans-complementation with the full length or interaction domain of ATP6V1A. Moreover, stably overexpressed ATP6V1A enhanced RABV growth in Vero cells, which are used for the production of rabies vaccine. Our findings identify a new partner for RABV M proteins and establish a new role of AT...Continue Reading

References

Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A Aruffo, B Seed
Nov 1, 1988·Reviews of Infectious Diseases·M Fekadu
May 2, 1997·The Journal of Biological Chemistry·Q LiuM Forgac
May 20, 1999·Molecular Membrane Biology·Y GaudinR Ruigrok
May 29, 2003·The Journal of General Virology·Stefan FinkeKarl-Klaus Conzelmann
Apr 5, 2005·Journal of Neurovirology·Monique Lafon
Oct 4, 2007·Nature Reviews. Molecular Cell Biology·Michael Forgac
Aug 12, 2008·Proceedings of the National Academy of Sciences of the United States of America·Chen Chen, Xiaowei Zhuang
Apr 29, 2009·Expert Review of Vaccines·P Noel BarrettM Keith Howard
Dec 1, 2009·Nature Reviews. Microbiology·Matthias J SchnellAmy Papaneri
May 24, 2011·Advances in Virus Research·Alan C Jackson
Jul 26, 2011·Journal of Structural Biology·Paul GuichardSergio Marco
Nov 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Debasis PandaAsit K Pattnaik
Feb 23, 2012·Viruses·Aurélie A V AlbertiniYves Gaudin
Aug 24, 2013·Journal of Virology·Silvia PiccinottiSean P J Whelan
Nov 16, 2013·Nucleic Acids Research·Sandra OrchardHenning Hermjakob
Mar 19, 2014·Vaccine·Diego FontanaClaudio Prieto
Oct 29, 2014·Expert Review of Vaccines·Manpreet KaurRakesh Bhatnagar
Jun 10, 2015·Proceedings of the National Academy of Sciences of the United States of America·Brent A HackettSara Cherry
Jul 19, 2016·Experimental Cell Research·Jie ZanJi-Yong Zhou
Jul 28, 2016·Scientific Reports·Colleen M GarveyShannon M Mumenthaler
Sep 11, 2016·Advanced Drug Delivery Reviews·Chensu WangJinming Gao
Oct 13, 2016·Current Infectious Disease Reports·Alan C Jackson
Dec 24, 2017·Research in Veterinary Science·Tinghua HuangMin Yao
Feb 2, 2018·Journal of Biomolecular Structure & Dynamics·Larisa V KordyukovaOleg V Batishchev
Mar 4, 2018·Molecular Therapy. Nucleic Acids·Shanxin PengSongdong Meng
Nov 6, 2018·Nucleic Acids Research·Yasset Perez-RiverolJuan Antonio Vizcaíno

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