The attachment process and physiological properties of Escherichia coli O157:H7 on quartz.

BMC Microbiology
Liliang WangQiaoyun Huang

Abstract

Manure application and sewage irrigation release many intestinal pathogens into the soil. After being introduced into the soil matrix, pathogens are commonly found to attach to soil minerals. Although the survival of mineral-associated Escherichia coli O157:H7 has been studied, a comprehensive understanding of the attachment process and physiological properties after attachment is still lacking. In this study, planktonic and attached Escherichia coli O157:H7 cells on quartz were investigated using RNA sequencing (RNA-seq) and the isobaric tagging for relative and absolute quantitation (iTRAQ) proteomic method. Based on the transcriptomic and proteomic analyses and gene knockouts, functional two-component system pathways were required for efficient attachment; chemotaxis and the Rcs system were identified to play determinant roles in E. coli O157:H7 attachment on quartz. After attachment, the pyruvate catabolic pathway shifted from the tricarboxylic acid (TCA) cycle toward the fermentative route. The survival rate of attached E. coli O157:H7 increased more than 10-fold under penicillin and vancomycin stress and doubled under alkaline pH and ferric iron stress. These results contribute to the understanding of the roles of chemota...Continue Reading

References

Mar 4, 2000·Applied and Environmental Microbiology·J V Gagliardi, J S Karns
Feb 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Karen Otto, Thomas J Silhavy
Apr 27, 2002·Applied and Environmental Microbiology·Xiuping JiangMichael P Doyle
Jul 17, 2003·Molecular Microbiology·Anne Francez-CharlotKaymeuang Cam
May 5, 2005·Applied and Environmental Microbiology·Abram AertsenChris W Michiels
Jun 21, 2005·BMC Microbiology·Gabriella Kiskó, Sibel Roller
Dec 13, 2005·Journal of Molecular Biology·Shuying WangDavid B McKay
Dec 24, 2009·Molecular Systems Biology·Natsuko YamamotoHirotada Mori
Aug 19, 2010·The EMBO Journal·Victor Sourjik, Judith P Armitage
Feb 2, 2011·Nature Reviews. Microbiology·Steven L PorterJudith P Armitage
Nov 19, 2011·FEMS Microbiology Letters·Stefanie L Vogt, Tracy L Raivio
Dec 21, 2011·Applied Microbiology and Biotechnology·S W PolyakG W Booker
Jan 17, 2012·Applied and Environmental Microbiology·Ryan C FinkFrancisco Diez-Gonzalez
Jan 26, 2013·Environmental Science & Technology·Peng CaiSharon L Walker
May 23, 2013·Environmental Science & Technology·Meagan S MauterMoshe Herzberg
Oct 24, 2013·Environmental Microbiology·Esteban Martínez-GarcíaVíctor de Lorenzo
Jun 5, 2014·Trends in Microbiology·Robert Belas
Apr 11, 2015·Annali Dell'Istituto Superiore Di Sanità·Raffaella ScottiRoberta Gabbianelli
Apr 26, 2015·Journal of Hazardous Materials·Zhiyuan YaoJianming Xu
Apr 26, 2015·Journal of Experimental Botany·Sara TrevisanSilvia Quaggiotti
Dec 27, 2015·Colloids and Surfaces. B, Biointerfaces·Hongjuan BaiEdvina Lamy
Jun 4, 2016·Colloids and Surfaces. B, Biointerfaces·Shams Tabrez KhanAbdulaziz A Al-Khedhairy
Mar 23, 2017·Colloids and Surfaces. B, Biointerfaces·Houzhen WeiZhenze Li
Oct 24, 2017·International Journal of Environmental Research and Public Health·Taoxiang ZhangWenhao Yang
Jan 6, 2018·Colloids and Surfaces. B, Biointerfaces·Carolin DewaldKlaus D Jandt
Apr 7, 2018·Colloids and Surfaces. B, Biointerfaces·Heejeong Lee, Dong Gun Lee
Sep 1, 2018·Colloids and Surfaces. B, Biointerfaces·Yongguang GuanQin Wang

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJNA511623

Methods Mentioned

BETA
RNA-seq
environmental stress
protein folding
gene knockout
PCR
quartz crystal microbalance
Assay

Software Mentioned

Mascot
Beacon Designer
SOAP2
SPSS
Primer

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of the American Veterinary Medical Association
C R Dorn
The New England Journal of Medicine
R L Guerrant, D M Theno
CMAJ : Canadian Medical Association Journal = Journal De L'Association Medicale Canadienne
E Weir
The New England Journal of Medicine
M J RyanT Cheasty
© 2021 Meta ULC. All rights reserved