The autodecomposition of radiolabeled human growth hormone

Journal of Immunoassay
G Baumann, K D Amburn

Abstract

Human growth hormone (hGH) was radiolabeled with 125I, using a gentle lactoperoxidase technique. The stability and decomposition products of this tracer were studied by frequent periodic analysis by Sephadex G-100 chromatography on a long column. Monomeric 125I-hGH showed an exponential decline, with a half-life of 61 days. The main radioactive degradation product was iodide, which appeared with a fractional appearance rate of 0.01136 per day. Secondary degradation products were a series of radioactive oligomers of hGH, which appeared with an overall fractional rate of 0.00525 per day. The kinetic data obtained should provide guidelines for the shelf-life and repurification schedule of radioiodinated polypeptides.

References

Jan 1, 1986·The Journal of Clinical Endocrinology and Metabolism·G BaumannB C DeVries
Dec 28, 1971·Biochimica Et Biophysica Acta·J I Thorell, B G Johansson

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Citations

Nov 26, 2008·Journal of Pharmaceutical Sciences·Shyeilla V DhuriaWilliam H Frey
Jun 19, 2004·Analytical Biochemistry·Kimberly A KellyLee Josephson
Jan 1, 1990·Acta Paediatrica Scandinavica. Supplement·G Baumann
Jul 17, 1987·Journal of Chromatography·J D PearsonD B DeWald

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