The autoimmunogenic chemicals HgCl2 and diphenylhydantoin stimulate IgG production to TNP-Ficoll and TNP-OVA, supporting and extending the graft-versus-host hypothesis for chemical induction of autoimmunity

Immunology
R AlbersN Bloksma

Abstract

Bypass of T-cell tolerance via non-cognate graft-versus-host (GVH)-like help from T-helper (Th) cells activated by chemically altered or induced epitopes, has been postulated as a mechanism underlying chemical induction of autoimmunity. To functionally test this hypothesis, we assessed whether the autoimmunogenic chemicals HgCl2 and diphenylhydantoin (DPH), like GVH reactions, stimulate specific immunoglobulin G (IgG) responses to trinitrophenyl (TNP)-Ficoll but not to TNP-ovalbumin. IgG responses were quantified in the popliteal lymph node by enzyme-linked immunosorbent spot-forming cell assay (ELISPOT) assays 7 days after s.c. injection of antigens, parental cells, chemicals or combinations thereof into the footpad of semi-allogeneic F1 mice. Antigens, chemicals, or cells alone induced few TNP-specific IgG antibody-forming cell (AFC) compared with untreated mice. Co-injection of parental cells or chemicals with TNP-Ficoll stimulated the TNP-specific response per lymph node approximately 50- and approximately 40-fold, respectively. In contrast, the IgG response to TNP-ovalbumin could not be stimulated by GVH reactions, whereas HgCl2 and DPH dose-dependently increased this response up to approximately 25- and approximately 250-...Continue Reading

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Citations

May 16, 1998·European Journal of Immunology·R AlbersR Pieters
Oct 20, 1999·Methods : a Companion to Methods in Enzymology·R Pieters, R Albers
Oct 21, 1999·Toxicology and Applied Pharmacology·R AlbersR Pieters
Oct 30, 2016·Regulatory Toxicology and Pharmacology : RTP·Ni LinHongtao Jin
Feb 24, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·John B CareyFrank N van Pelt
Apr 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stefan NierkensRaymond Pieters
Jan 13, 2006·The Journal of Physical Chemistry. a·Hui-Chung Tai, Carmay Lim
Dec 21, 2004·Chemical Research in Toxicology·M PopovićJ Uetrecht

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