The autoradiographic distribution of substance P binding sites in guinea-pig vas deferens is altered by capsaicin pretreatment.

European Journal of Pharmacology
C J MussapE Burcher

Abstract

The distribution of binding sites for [125I]Bolton-Hunter substance P (BHSP) was investigated in vasa deferentia from normal, capsaicin-pretreated and vehicle-pretreated guinea-pigs, using qualitative and quantitative autoradiography. Dense binding of BHSP was seen over the outer longitudinal muscle with less over the inner longitudinal muscle. Very low specific binding occurred to the circular muscle and was absent in the mucosa. Characterization in slide-mounted sections showed that binding was saturable and of high affinity, with equilibrium dissociation constant (KD) 91 +/- 15 pM. BHSP was displaced by substance P greater than neurokinin A greater than neurokinin B, suggesting binding to NK-1 receptors. Capsaicin pretreatment had no effect on the lengthwise distribution of binding sites but significantly altered their relative distribution between the different smooth muscle layers. There was a very marked increase in BHSP binding over the inner longitudinal muscle and the inner part of the circular muscle layer, whereas binding was virtually abolished over the outer longitudinal muscle, compared with vehicle control. Capsaicin-sensitive binding sites over the outer longitudinal muscle may be located presynaptically on caps...Continue Reading

References

Jan 1, 1978·Neuroscience·P AlmF Sundler
Sep 24, 1985·European Journal of Pharmacology·P Le GrevesT Hökfelt
Nov 4, 1986·European Journal of Pharmacology·C M LeeL L Iversen
Jan 12, 1987·Life Sciences·D RegoliP D'Orléans-Juste
Oct 1, 1987·British Journal of Pharmacology·H MoritokiK Kitagawa
Oct 27, 1987·European Journal of Pharmacology·J A StephensonE Burcher
Dec 1, 1986·The Journal of Pharmacy and Pharmacology·R G Chess-WilliamsD J Sheridan
Sep 9, 1986·European Journal of Pharmacology·E Burcher, S H Buck
May 1, 1987·Journal of the Autonomic Nervous System·D B Hoover, J C Hancock
Mar 1, 1974·Acta Physiologica Scandinavica·U S von Euler, P Hedqvist
Mar 1, 1982·Naunyn-Schmiedeberg's Archives of Pharmacology·C M LeeB E Sandberg
Jan 28, 1983·European Journal of Pharmacology·S P WatsonL L Iversen
Sep 1, 1980·Analytical Biochemistry·P J Munson, D Rodbard
Aug 1, 1983·Computer Programs in Biomedicine·G A McPherson

❮ Previous
Next ❯

Citations

Jun 1, 1993·Journal of Neurochemistry·C J MussapE Burcher
Dec 14, 2004·Life Sciences·Luz CandenasJocelyn N Pennefather

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.