PMID: 508Aug 1, 1975

The avian erythrocyte: a study of fixation for electron microscopy

Journal of Microscopy
J N Brown


The quality of ultrastructural preservation of the avian erythrocyte achieved using various fixation techniques is evaluated. Different combinations of initial fixatives, buffers and post-fixation procedures were tested as well as variations in fixative osmolarity, pH and temperature. Of the commonly used initial fixatives (glutaraldehyde, acrolein and formaldehyde), 2% glutaraldehyde, alone in a slightly hypertonic buffer containing divalent ions, produced optimum erythrocyte preservation. The osmolarity was balanced using a non-electrolyte such as a sucrose. The addition of 12% hexylene glycol to the buffer solutions also improves erythrocyte preservation, as evidenced by the increased stability of the marginal microtubules, microfilaments and proteinaceous material. The use of Spurr low-viscosity epoxy resin enables the cells to be collected using low gravitational centrifugation.


Jun 1, 1971·The Journal of Cell Biology·Q Bone, E J Denton
Jul 1, 1971·Journal of Ultrastructure Research·J R Harris, J N Brown
Jul 1, 1972·The Histochemical Journal·D Hopwood
Jul 1, 1972·The Histochemical Journal·Q Bone, K P Ryan
Nov 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·P N McMillan, R B Luftig
May 1, 1967·British Journal of Haematology·D Kay, B J Cuddigan
Jul 1, 1967·Stain Technology·M D MaserC W Philpott
Jan 10, 1969·Science·J B Kirkpatrick
Aug 1, 1967·Journal of Bacteriology·C C RemsenS W Watson
Jun 20, 1972·Annals of the New York Academy of Sciences·David E Green
Feb 1, 1973·Journal of Ultrastructure Research·R J Tomanek, U KARLSSON
Sep 1, 1967·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H Warshawsky, G Moore
Mar 1, 1968·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H D Fahimi, P Drochmans
Dec 1, 1968·Journal of Ultrastructure Research·J Gil, E R Weibel
Jan 1, 1969·Journal of Ultrastructure Research·A R Spurr
Jan 1, 1969·International Review of Cytology·E R Weibel
Jun 1, 1969·Die Naturwissenschaften·W W FrankeH Samek
Jun 1, 1965·The Journal of Cell Biology·R E KANE, A Forer
Apr 1, 1970·Journal of Ultrastructure Research·O Behnke
Jan 1, 1971·The Journal of Cell Biology·F M MorelH Wayland
Aug 1, 1967·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P J Anderson
Sep 25, 1957·The Journal of Biophysical and Biochemical Cytology·J B CAULFIELD
Mar 1, 1961·The Journal of Biophysical and Biochemical Cytology·H G DAVIES
Sep 1, 1962·The Journal of Cell Biology·H G DAVIES, M SPENCER
Apr 1, 1964·Journal of Ultrastructure Research·T N TAHMISIAN
Sep 1, 1964·The Journal of Cell Biology·C FRANZINI-ARMSTRONG, K R Porter
May 6, 1965·Zeitschrift für Zellforschung und mikroskopische Anatomie·R M TORACK
Apr 1, 1965·The Journal of Cell Biology·R E KANE
Oct 1, 1963·The Journal of Cell Biology·M C Ledbetter, K R Porter
Nov 1, 1970·The Journal of Cell Biology·J B KirkpatrickP M Howley
Jun 1, 1972·Annals of the New York Academy of Sciences·David E Green


Jun 18, 2015·Biophysical Journal·Ruddi Rodríguez-GarcíaFrancisco Monroy

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