PMID: 508Aug 1, 1975

The avian erythrocyte: a study of fixation for electron microscopy

Journal of Microscopy
J N Brown

Abstract

The quality of ultrastructural preservation of the avian erythrocyte achieved using various fixation techniques is evaluated. Different combinations of initial fixatives, buffers and post-fixation procedures were tested as well as variations in fixative osmolarity, pH and temperature. Of the commonly used initial fixatives (glutaraldehyde, acrolein and formaldehyde), 2% glutaraldehyde, alone in a slightly hypertonic buffer containing divalent ions, produced optimum erythrocyte preservation. The osmolarity was balanced using a non-electrolyte such as a sucrose. The addition of 12% hexylene glycol to the buffer solutions also improves erythrocyte preservation, as evidenced by the increased stability of the marginal microtubules, microfilaments and proteinaceous material. The use of Spurr low-viscosity epoxy resin enables the cells to be collected using low gravitational centrifugation.

References

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Citations

Jun 18, 2015·Biophysical Journal·Ruddi Rodríguez-GarcíaFrancisco Monroy

Related Concepts

Aqualin
Ampholytes
Plasma Membrane
Cell Nucleus
Chloride Ion Level
Chromatin
Cytological Techniques
Erythrocytes
Formol
Sekumatic

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