The B chromosome of Sorghum purpureosericeum reveals the first pieces of its sequence.

Journal of Experimental Botany
Miroslava KarafiátováJan Bartoš

Abstract

More than a century has passed since the B chromosomes were first discovered. Today we know much of their variability, morphology, and transmission to plant progeny. With the advent of modern technologies, B chromosome research has accelerated, and some of their persistent mysteries have since been uncovered. Building on this momentum, here we extend current knowledge of B chromosomes in Sorghum purpureosericeum to the sequence level. To do this, we estimated the B chromosome size at 421 Mb, sequenced DNA from flow-sorted haploid pollen nuclei of both B-positive (B+) and B-negative (B0) plants, and performed a repeat analysis on the Illumina raw sequence data. This analysis revealed nine putative B-specific clusters, which were then used to develop B chromosome-specific markers. Additionally, cluster SpuCL4 was identified and verified to be a centromeric repeat. We also uncovered two repetitive clusters (SpuCL168 and SpuCL115), which hybridized exclusively on the B chromosome under fluorescence in situ hybridization and can be considered as robust cytogenetic markers. Given that B chromosomes in Sorghum are rather unstable across all tissues, our findings could facilitate expedient identification of B+ plants and enable a wide ...Continue Reading

Associated Datasets

References

Oct 1, 1993·Genetics·M R Alfenito, J A Birchler
Sep 26, 2002·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·A Masoudi-NejadT R Endo
Jan 24, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·J DolezelJ Greilhuber
Dec 15, 2004·Annals of Botany·H James PriceJ Spencer Johnston
Mar 9, 2005·Cytogenetic and Genome Research·A KatoJ A Birchler
Mar 24, 2007·Bioinformatics·Triinu Koressaar, Maido Remm
Jul 17, 2007·Genome Génome / Conseil National De Recherches Canada·Wayne R Carlson
Sep 15, 2007·Nature Protocols·Jaroslav DolezelJan Suda
May 28, 2008·Cytogenetic and Genome Research·R N JonesM J Puertas
Dec 8, 2009·Science·Patrick S SchnableRichard K Wilson
Jun 26, 2012·Nucleic Acids Research·Andreas UntergasserSteven G Rozen
Jul 27, 2012·Cytogenetic and Genome Research·R E Masonbrink, J A Birchler
Aug 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Mihaela Maria MartisAndreas Houben
Sep 13, 2013·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·M KarafiátováJ Doležel
Aug 26, 2016·Chromosoma·Guilherme T ValenteCesar Martins
Jul 26, 2017·The New Phytologist·Ugo D'AmbrosioSònia Garcia
May 24, 2018·Nucleic Acids Research·Enis AfganDaniel Blankenberg
Oct 3, 2018·Genes·Handong SuFangpu Han
Feb 20, 2019·Cells·Syed Farhan Ahmad, Cesar Martins
Jul 11, 2019·The Plant Genome·Mahmoud SaidJan Vrána
Dec 29, 2019·Scientific Reports·Rahman EbrahimzadeganGhader Mirzaghaderi

❮ Previous
Next ❯

Citations

Apr 4, 2021·Plants·Martina BednářováJan Bartoš
May 14, 2021·BMC Genomics·Jordana Inácio Nascimento-OliveiraCesar Martins

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
PCR

Software Mentioned

Galaxy
RepeatExplorer
RepeatExplorer2
GeneRuler
TAREN
Primer3
TAREAN

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.