The B Subunit of PirABvp Toxin Secreted from Vibrio parahaemolyticus Causing AHPND Is an Amino Sugar Specific Lectin

Pathogens
Marcelo Victorio-De Los SantosPatricia Cano-Sánchez

Abstract

Vibrio parahaemolyticus (Vp) is the etiological agent of the acute hepatopancreatic necrosis disease (AHPND) in Penaeus vannamei shrimp. Vp possesses a 63-70 kb conjugative plasmid that encodes the binary toxin PirA vp /PirB vp . The 250 kDa PirAB vp complex was purified by affinity chromatography with galactose-sepharose 4B and on a stroma from glutaraldehyde-fixed rat erythrocytes column, as a heterotetramer of PirA vp and PirB vp subunits. In addition, recombinant pirB (rPirB vp ) and pirA (rPirA vp ) were obtained. The homogeneity of the purified protein was determined by SDS-PAGE analysis, and the yield of protein was 488 ng/100 μg of total protein of extracellular products. The PirAB vp complex and the rPirB vp showed hemagglutinating activity toward rat erythrocytes. The rPirA vp showed no hemagglutinating capacity toward the animal red cells tested. Among different mono and disaccharides tested, only GalNH2 and GlcNH2 were able to inhibit hemagglutination of the PirAB vp complex and the rPirB vp . Glycoproteins showed inhibitory specificity, and fetuin was the glycoprotein that showed the highest inhibition. Other glycoproteins, such as mucin, and glycosaminoglycans, such as heparin, also inhibited the activity. ...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1982·Advances in Carbohydrate Chemistry and Biochemistry·R Schauer
Apr 1, 1982·Infection and Immunity·L F Hanne, R A Finkelstein
Mar 15, 1980·The Biochemical Journal·J Schlepper-SchäferH Kolb
Mar 7, 1995·Biochemistry·D P Mascotti, T M Lohman
Apr 8, 2000·Current Opinion in Structural Biology·F B SheinermanB Honig
Jun 1, 2000·Journal of the American Society for Mass Spectrometry·D J HarveyI B Wilson
Jul 13, 2000·Journal of Biochemical and Biophysical Methods·G A Fragkiadakis
Sep 1, 2000·FEMS Immunology and Medical Microbiology·N Gilboa-GarberN C Garber
Nov 18, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·R ZentenoE Zenteno
Nov 9, 2002·Biochimie·Yvon Michel-Briand, Christine Baysse
Jan 21, 2003·Journal of Bacteriology·Annabel H A ParretRené De Mot
Jul 2, 2004·Glycobiology·Nathan Sharon, Halina Lis
Sep 10, 2005·Applied and Environmental Microbiology·Annabel H A ParretRené De Mot
Dec 15, 2005·Zoology : Analysis of Complex Systems, ZACS·Roland Schauer
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Alessandro OriDavid Garth Fernig
Apr 2, 2011·The Journal of Biological Chemistry·Alessandro OriDavid G Fernig
Jul 1, 2010·Toxins·Oludare OdumosuWilliam Langridge
Nov 28, 2012·Biochemical Society Transactions·Maarten G K GhequireRené De Mot
Mar 8, 2013·PLoS Pathogens·Maarten G K GhequireRené De Mot
May 9, 2013·Journal of Molecular Modeling·Joseph AbhilashMadhathilkovilakath Haridas
Mar 13, 2014·Annual Review of Biochemistry·Ding Xu, Jeffrey D Esko
Jun 14, 2014·FEMS Microbiology Reviews·Maarten G K Ghequire, René De Mot
Aug 15, 2014·Methods in Molecular Biology·Els J M Van Damme
Aug 22, 2014·Diseases of Aquatic Organisms·Linda NunanSilvia Gomez-Jimenez
Apr 8, 2015·Developmental and Comparative Immunology·Hui YangJianhai Xiang
Aug 12, 2015·Proceedings of the National Academy of Sciences of the United States of America·Chung-Te LeeChu-Fang Lo
Oct 5, 2015·Trends in Microbiology·Maarten G K Ghequire, René De Mot
Oct 28, 2015·Diseases of Aquatic Organisms·Leobert D de la PeñaCynthia P Saloma
May 11, 2017·Malaria Journal·Casper HempelTrine Staalsø
May 27, 2017·Fish & Shellfish Immunology·Efrén García-MaldonadoAlejandra Hernández-Santoyo

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
affinity purification

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.