PMID: 8990160Jan 7, 1997Paper

The B12-dependent ribonucleotide reductase from the archaebacterium Thermoplasma acidophila: an evolutionary solution to the ribonucleotide reductase conundrum

Proceedings of the National Academy of Sciences of the United States of America
A Tauer, S A Benner

Abstract

A coenzyme B12-dependent ribonucleotide reductase was purified from the archaebacterium Thermoplasma acidophila and partially sequenced. Using probes derived from the sequence, the corresponding gene was cloned, completely sequenced, and expressed in Escherichia coli. The deduced amino acid sequence shows that the catalytic domain of the B12-dependent enzyme from T. acidophila, some 400 amino acids, is related by common ancestry to the diferric tyrosine radical iron(III)-dependent ribonucleotide reductase from E. coli, yeast, mammalian viruses, and man. The critical cysteine residues in the catalytic domain that participate in the thiyl radical-dependent reaction have been conserved even though the cofactor that generates the radical is not. Evolutionary bridges created by the T. acidophila sequence and that of a B12-dependent reductase from Mycobacterium tuberculosis establish homology between the Fe-dependent enzymes and the catalytic domain of the Lactobacillus leichmannii B12-dependent enzyme as well. These bridges are confirmed by a predicted secondary structure for the Lactobacillus enzyme. Sequence similarities show that the N-terminal domain of the T. acidophila ribonucleotide reductase is also homologous to the anaerob...Continue Reading

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Citations

Jun 7, 2003·Advances in Enzyme Regulation·Steven A Benner
Oct 8, 1998·Advances in Enzyme Regulation·S A BennerD Schreiber
Feb 14, 2002·Progress in Biophysics and Molecular Biology·H EklundP Nordlund
Feb 20, 1998·Current Opinion in Genetics & Development·P Forterre
Feb 13, 2001·Trends in Biochemical Sciences·J StubbeC S Yee
Nov 18, 1998·Current Opinion in Chemical Biology·J A Gerlt, P C Babbitt
Aug 14, 1999·Nucleic Acids Research·D D LeipeE V Koonin
Dec 18, 2002·Journal of Bacteriology·J D WoodsonJ C Escalante-Semerena
Aug 20, 2005·Journal of Bacteriology·Jesse D WoodsonJorge C Escalante-Semerena
May 5, 2006·Journal of Bacteriology·Nicole R BuanJorge C Escalante-Semerena
Oct 6, 1998·Annual Review of Biochemistry·A Jordan, P Reichard
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·J RieraM Fontecave
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·A JordanP Reichard
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·J Stubbe
Nov 27, 2014·Journal of Molecular Evolution·Anthony M PooleJun Ogawa
Nov 27, 1999·Structure·H Eklund, M Fontecave
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Nov 2, 2012·Pharmaceuticals·Sanath R WijerathnaChris G Dealwis
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Mar 19, 2005·Molecular Biology and Evolution·Anthony M Poole, Derek T Logan
Sep 13, 2002·Progress in Biophysics and Molecular Biology·Brian K Davis
Feb 19, 2002·Chemical Reviews·JoAnne Stubbe, Wilfred A. van Der Donk
Jan 28, 1999·Biochemical and Biophysical Research Communications·D L GerloffS A Benner
Sep 4, 2003·Journal of the American Chemical Society·Kenneth M DollRichard G Finke

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