PMID: 9555905Apr 29, 1998Paper

The Bacillus subtilis DinR binding site: redefinition of the consensus sequence

Journal of Bacteriology
K W WinterlingR Woodgate

Abstract

Recently, the DinR protein was established as the cellular repressor of the SOS response in the bacterium Bacillus subtilis. It is believed that DinR functions as the repressor by binding to a consensus sequence located in the promoter region of each SOS gene. The binding site for DinR is believed to be synonymous with the formerly identified Cheo box, a region of 12 bp displaying dyad symmetry (GAAC-N4-GTTC). Electrophoretic mobility shift assays revealed that highly purified DinR does bind to such sites located upstream of the dinA, dinB, dinC, and dinR genes. Furthermore, detailed mutational analysis of the B. subtilis recA operator indicates that some nucleotides are more important than others for maintaining efficient DinR binding. For example, nucleotide substitutions immediately 5' and 3' of the Cheo box as well as those in the N4 region appear to affect DinR binding. This data, combined with computational analyses of potential binding sites in other gram-positive organisms, yields a new consensus (DinR box) of 5'-CGAACRNRYGTTYC-3'. DNA footprint analysis of the B. subtilis dinR and recA DinR boxes revealed that the DinR box is centrally located within a DNA region of 31 bp that is protected from hydroxyl radical cleavag...Continue Reading

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Citations

Dec 31, 2003·The Journal of Biological Chemistry·Carmelo BisognanoPierre Vaudaux
Aug 4, 2005·Journal of Bacteriology·Jordi CuñéJordi Barbe
Jul 21, 2005·Journal of Bacteriology·Susana CampoyJordi Barbé
Sep 22, 2007·FEMS Microbiology Reviews·Ivan ErillJordi Barbé
Jul 17, 2007·Applied and Environmental Microbiology·Johannes SjöholmPeter Lindblad
Apr 26, 2008·Molecular Genetics and Genomics : MGG·Mei-Kwei YangVin-Long Sung
Oct 16, 2007·Journal of Bacteriology·Marc AbellaJordi Barbé
Sep 18, 2012·Journal of Bacteriology·Dana GebhartDean Scholl
Mar 11, 2005·Microbiology·Hannes NahrstedtFriedhelm Meinhardt
Oct 11, 2002·Journal of Bacteriology·Antonio R Fernández de HenestrosaJordi Barbé
Jun 22, 2007·Molecular Microbiology·Carles UbedaJosé R Penadés
Sep 28, 2006·Applied Microbiology and Biotechnology·Jens WaldeckFriedhelm Meinhardt
Nov 4, 2005·Journal of Bacteriology·Nora AuCharles M Lovett
Dec 4, 2003·Molecular Genetics and Genomics : MGG·G MazónJ Barbé
Oct 2, 2007·Journal of Bacteriology·Jonathan LivnyMatthew K Waldor
Aug 5, 2003·Journal of Bacteriology·Pablo CastánJosé Berenguer
Jul 8, 2005·Applied and Environmental Microbiology·Mei-Kwei YangVin-Long Sung
Aug 31, 2012·Microbiology and Molecular Biology Reviews : MMBR·Justin S LenhartLyle A Simmons
Jun 14, 2014·FEMS Microbiology Reviews·Zeynep Baharoglu, Didier Mazel
Jul 21, 2017·Clinical Microbiology Reviews·Elisabeth HodilleOana Dumitrescu
Jul 17, 2003·Molecular Microbiology·Susana CampoyJordi Barbé
Dec 12, 2001·Microbiology·K G PapavinasasundaramE O Davis
Aug 10, 2006·Antonie van Leeuwenhoek·Gerard MazónJordi Barbé
Sep 26, 2001·Applied and Environmental Microbiology·S NuytsJ Anné
Apr 12, 2000·Molecular Microbiology·A R Fernández De HenestrosaR Woodgate
Feb 13, 1999·FEMS Microbiology Letters·M LabaziJ Barbé
Nov 6, 2004·Microbiology·Gerard MazónJordi Barbé

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