PMID: 8969214Dec 27, 1996Paper

The bacillus subtilis dinR gene codes for the analogue of Escherichia coli LexA. Purification and characterization of the DinR protein.

The Journal of Biological Chemistry
M C MillerCharles M Lovett

Abstract

The Bacillus subtilis dinR gene encodes a 23-kDa protein that shares about 34% homology with the Escherichia coli LexA protein. We have purified the dinR gene product to near homogeneity, and we describe its activities. The purified DinR protein binds specifically to the promoter regions of three B. subtilis SOS genes: dinB, dinC, and recA. Electrophoretic mobility of DinR-promoter complexes in each case is identical to that of promoters bound by the B. subtilis SOS repressor (Lovett, et al., (1993) J. Bacteriol. 175, 6842-6849). Analysis of hydroxyl radical footprints of DinR bound to the dinC promoter indicates that DinR interacts with one side of the DNA providing access to the consensus operator site (5'-GAACN4GTTC-3') within two adjacent major grooves. Consistent with its proposed role as a transcriptional repressor, purified DinR displaces B. subtilis RNA polymerase from the recA promoter and represses transcription of the recA gene in vitro. We also show that purified DinR protein undergoes general base-catalyzed autodigestion as well as RecA-mediated cleavage at the peptide bond between Ala-91 and Gly-92. Corresponding to its cleavage by activated RecA following DNA damage, the level of DinR is significantly reduced in ...Continue Reading

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Citations

Jul 15, 1998·Molecular Microbiology·A R Fernández de HenestrosaJ Barbé
Sep 5, 2001·Molecular Microbiology·C W PriceP Youngman
Oct 9, 2002·Molecular Microbiology·Céline FabretPhilippe Noirot
Nov 2, 2002·Molecular Microbiology·Elaine O DavisErik C Böttger
Nov 5, 2005·Nucleic Acids Research·Eli S GrobanCharles M Lovett
Jul 15, 2006·Nucleic Acids Research·Laura M Patterson-FortinGeorge W Owttrim
Jul 8, 2005·Applied and Environmental Microbiology·Mei-Kwei YangVin-Long Sung
Sep 6, 2011·Journal of Bacteriology·Nadine FornelosJacques Mahillon
Nov 4, 2005·Journal of Bacteriology·Nora AuCharles M Lovett
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Jan 5, 2002·Clinical Chemistry and Laboratory Medicine : CCLM·P A IacovazziM Correale
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Sep 3, 2008·Molecular Microbiology·Baundauna BoseAlan D Grossman
Jun 16, 2011·Proteomics·Rebecca SchroeterThomas Schweder
Sep 3, 2010·Molecular Microbiology·Remi BernardDavid Z Rudner
Jun 29, 2011·Journal of Molecular Biology·Vicki L ColledgeAnthony J Wilkinson
Jan 31, 2018·Journal of Bacteriology·Laura C BohorquezLeendert W Hamoen
Jun 17, 2008·FEBS Letters·Laura M Patterson-Fortin, George W Owttrim
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Dec 12, 2001·Microbiology·K G PapavinasasundaramE O Davis

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