PMID: 2498284Jun 1, 1989Paper

The Bacillus subtilis flagellin gene (hag) is transcribed by the sigma 28 form of RNA polymerase

Journal of Bacteriology
D B Mirel, M J Chamberlin

Abstract

The Bacillus subtilis gene hag, which codes for the flagellin structural protein, was identified by DNA sequence analysis in a collection of DNA fragments bearing in vitro promoters for the sigma 28 form of RNA polymerase. The hag gene and adjacent regions of the B. subtilis chromosome were restriction mapped, and the nucleotide sequence was determined. The hag gene was transcribed at all stages of growth from a single promoter that had sequences in the promoter recognition region characteristic of the consensus sequence for the sigma 28 holoenzyme. Transcription of hag was eliminated by insertion mutations that blocked synthesis of the sigma 28 protein. These findings provide strong support for the previous proposal that the sigma 28 form of RNA polymerase controls transcription of a regulon specifying flagellar, chemotaxis, and motility functions in B. subtilis (J. D. Helmann and M. J. Chamberlin, Proc. Natl. Acad. Sci. USA 84:6422-6424, 1987). The steady-state levels of hag mRNA increased during exponential growth and peaked as the B. subtilis cells entered the stationary phase. The transcript levels then decreased to zero within 4 h after the onset of sporulation. Hence, sigma 28 RNA polymerase function is temporally regula...Continue Reading

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J A JaehningM J Chamberlin
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Nov 1, 1987·Journal of Bacteriology·S M LoganT J Trust
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D N Arnosti, M J Chamberlin
Apr 1, 1988·Journal of Bacteriology·J D HelmannM J Chamberlin
Jun 1, 1985·Microbiological Reviews·P J Piggot, J A Hoch
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C BinnieR Losick
Jan 1, 1986·Annual Review of Genetics·R LosickP J Piggot
Apr 1, 1988·Journal of Bacteriology·J D HelmannM J Chamberlin
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J D Helmann, M J Chamberlin
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S A Minnich, A Newton
Dec 1, 1986·Journal of Bacteriology·Y Komeda
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·R J DelangeA N Glazer
Jun 28, 1968·Journal of Molecular Biology·R J MartinezS R Tronick
Jun 13, 1966·Biochemical and Biophysical Research Communications·D T Denhardt
Dec 1, 1984·Journal of Bacteriology·H M Pooley, D Karamata
May 1, 1984·Analytical Biochemistry·J Meinkoth, G Wahl
Apr 1, 1981·Journal of Bacteriology·E FerrariJ A Hoch
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J L WiggsM J Chamberlin
Jul 23, 1982·Science·S L McKnight, R Kingsbury
Jun 1, 1983·Microbiological Reviews·B J Bachmann
May 25, 1984·Nucleic Acids Research·V Brendel, E N Trifonov
May 1, 1961·Journal of Bacteriology·C Anagnostopoulos, J Spizizen
Oct 15, 1958·Proceedings of the National Academy of Sciences of the United States of America·J Spizizen

❮ Previous
Next ❯

Citations

Jan 1, 1995·Antonie van Leeuwenhoek·P J NuijtenB A van der Zeijst
Feb 23, 1999·Molecular Microbiology·C A Murphy, R Belas
Jul 17, 2003·Molecular Microbiology·Daniel B Kearns, Richard Losick
Aug 2, 2006·Molecular Microbiology·David Dubnau, Richard Losick
Feb 23, 2012·Applied Microbiology and Biotechnology·Christos P PapaneophytouDimitrios A Kyriakidis
Aug 2, 2012·Infection and Immunity·Annie AubrySusan M Logan
Sep 25, 2014·Annual Review of Genetics·Sampriti Mukherjee, Daniel B Kearns
Apr 20, 2019·Cell Stress & Chaperones·Shaomin Yan, Guang Wu
Sep 1, 1992·Molecular Microbiology·P B CarpenterG W Ordal
Jul 23, 2003·Journal of Food Protection·Linda C Kelana, Mansel W Griffiths
Mar 18, 2010·Molecular Microbiology·Loralyn M Cozy, Daniel B Kearns
Jul 9, 2011·Molecular Microbiology·Yi-Huang HsuehDaniel B Kearns
Oct 15, 2014·Journal of Bacteriology·Rebecca A Calvo, Daniel B Kearns
Mar 26, 2016·Frontiers in Microbiology·Noël MolièreKürşad Turgay
Apr 21, 2019·Applied and Environmental Microbiology·Ariel OgranIlana Kolodkin-Gal
Apr 1, 1996·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·P MargotD Karamata
Jun 26, 2012·Journal of Bacteriology·Colleen R CourtneyDaniel B Kearns
Nov 5, 2013·Journal of Bacteriology·Carol Vargas-BautistaPaul Straight
Oct 22, 2014·Journal of Bacteriology·Qutaiba O Ababneh, Jennifer K Herman
Jul 5, 2013·Molecular Genetics and Genomics : MGG·Adam IwanickiMichał Obuchowski
Aug 6, 2015·PLoS Genetics·Andrew M PhillipsDaniel B Kearns
Oct 25, 2017·Journal of Bacteriology·Ashley N HallDaniel B Kearns
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·K Fredrick, J D Helmann
Jul 19, 2014·Bioscience, Biotechnology, and Biochemistry·Hiraku TakadaHirofumi Yoshikawa
May 21, 2019·Frontiers in Microbiology·Lisa M LeeShmuel M Rubinstein
May 3, 2002·Proteomics·Haike AntelmannMichael Hecker
Nov 10, 2009·Canadian Journal of Microbiology·Prashant Kodgire, K Krishnamurthy Rao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.