The Bacillus subtilis phoAIV gene: effects of in vitro inactivation on total alkaline phosphatase production

Gene
N KappF M Hulett

Abstract

A degenerative oligodeoxyribonucleotide probe deduced from the first 19 amino acids of the mature alkaline phosphatase IV (APase IV) protein was used to clone a DNA fragment internal to the coding region of the phoAIV gene of Bacillus subtilis. An insertional mutation was constructed in the phoAIV locus using the integrative plasmid, pJM103, containing the cloned DNA fragment. The strain with the interrupted phoAIV gene showed no detectable APase IV product on Western-blot analysis. The impact of the phoAIV interruption on total APase production in B. subtilis 168 was analyzed under both phosphate starvation and sporulation culturing conditions. The mutation in phoAIV reduced total APase-specific activity by 75% in phosphate-starved cells, and resulted in the elimination of a salt-extractable membrane APase, as well as the secreted APase IV. Analysis of this membrane APase indicated that it is a phoAIV gene product which is localized within the membrane fraction of the lysed cell and not secreted. There was no effect on the production of sporulation APase. The phoAIV::pJM103 insertion was mapped and determined to be located at approx. 73 degrees on the B. subtilis 360 degrees chromosome.

References

Aug 1, 1975·Biulleten' eksperimental'noĭ biologii i meditsiny·V I Korinteli, V M Kushnarev
Apr 1, 1977·Applied and Environmental Microbiology·R A DedonderF Kunst
Feb 1, 1990·Journal of Bacteriology·F M HulettK Jensen
Nov 1, 1989·Molecular Microbiology·R GrossR Rappuoli
Jun 1, 1985·Microbiological Reviews·P J Piggot, J A Hoch
Aug 1, 1986·Journal of Bacteriology·M A Vandeyar, S A Zahler
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B T NixonF M Ausubel
Dec 1, 1988·Journal of Bacteriology·T SekiH Saito
Jun 1, 1974·Journal of General Microbiology·W D Grant
Nov 15, 1973·European Journal of Biochemistry·J C Le Hégarat, C Anagnostopoulos
Sep 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·P SchaefferJ P Aubert
May 1, 1984·Analytical Biochemistry·J Meinkoth, G Wahl
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S M Harley, H Beevers

❮ Previous
Next ❯

Citations

Jun 6, 2003·Preparative Biochemistry & Biotechnology·Dong YangLijia An
Jan 1, 1993·Critical Reviews in Biotechnology·K Murata
Mar 1, 1997·Microbiology·Jörg P MülerColin R Harwood

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.