The Bacillus subtilis tyrZ gene encodes a highly selective tyrosyl-tRNA synthetase and is regulated by a MarR regulator and T box riboswitch

Journal of Bacteriology
Rebecca N Williams-WagnerTina M Henkin

Abstract

Misincorporation of D-tyrosine (D-Tyr) into cellular proteins due to mischarging of tRNA(Tyr) with D-Tyr by tyrosyl-tRNA synthetase inhibits growth and biofilm formation of Bacillus subtilis. Furthermore, many B. subtilis strains lack a functional gene encoding D-aminoacyl-tRNA deacylase, which prevents misincorporation of D-Tyr in most organisms. B. subtilis has two genes that encode tyrosyl-tRNA synthetase: tyrS is expressed under normal growth conditions, and tyrZ is known to be expressed only when tyrS is inactivated by mutation. We hypothesized that tyrZ encodes an alternate tyrosyl-tRNA synthetase, expression of which allows the cell to grow when D-Tyr is present. We show that TyrZ is more selective for L-Tyr over D-Tyr than is TyrS; however, TyrZ is less efficient overall. We also show that expression of tyrZ is required for growth and biofilm formation in the presence of D-Tyr. Both tyrS and tyrZ are preceded by a T box riboswitch, but tyrZ is found in an operon with ywaE, which is predicted to encode a MarR family transcriptional regulator. Expression of tyrZ is repressed by YwaE and also is regulated at the level of transcription attenuation by the T box riboswitch. We conclude that expression of tyrZ may allow growth...Continue Reading

References

Jan 1, 1991·DNA Sequence : the Journal of DNA Sequencing and Mapping·P GlaserR Dedonder
Oct 25, 1990·Nucleic Acids Research·T D Schneider, R M Stephens
Dec 1, 1974·Journal of Virology·R E Yasbin, F E Young
May 28, 1967·Journal of Molecular Biology·R Calendar, P Berg
Jan 1, 1972·Molecular & General Genetics : MGG·R C Gethmann
Apr 1, 1969·Journal of Bacteriology·W S Champney, R A Jensen
Dec 1, 1970·Journal of General Microbiology·D W TempestC M Brown
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·R G Martin, J L Rosner
Mar 8, 2000·Microbiology and Molecular Biology Reviews : MMBR·C R WoeseD Söll
Apr 15, 2000·The Journal of Biological Chemistry·J SoutourinaP Plateau
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·S S BrandaR Kolter
Mar 20, 2003·FEMS Microbiology Letters·Kei AsaiYoshito Sadaie
Aug 19, 2003·RNA·Mary R YousefTina M Henkin
Jan 15, 1961·Proceedings of the National Academy of Sciences of the United States of America·E W NESTER, J LEDERBERG
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Jun 4, 2004·Journal of Bacteriology·Steven S BrandaRoberto Kolter
Aug 5, 2004·The Journal of Biological Chemistry·Olga SoutourinaPierre Plateau
Sep 28, 2004·The Journal of Biological Chemistry·Steven P Wilkinson, Anne Grove
Apr 28, 2005·Molecular Microbiology·Torsten Stein
May 1, 1961·Journal of Bacteriology·C Anagnostopoulos, J Spizizen
Jul 1, 1965·Journal of Bacteriology·J N Aronson, G R Wermus
Apr 22, 2009·Annual Review of Microbiology·Jiqiang LingMichael Ibba
Sep 19, 2009·Science·Hubert LamMatthew K Waldor
May 1, 2010·Science·Ilana Kolodkin-GalRichard Losick
Aug 19, 2010·Journal of Molecular Cell Biology·Inoka C Perera, Anne Grove
Dec 17, 2010·Cellular and Molecular Life Sciences : CMLS·Felipe CavaMatthew K Waldor
May 8, 2013·Antimicrobial Agents and Chemotherapy·Jose Antonio EscuderoBruno González-Zorn
Jul 6, 2013·International Journal of Medical Microbiology : IJMM·Felipe Lopes TeixeiraLeandro Araujo Lobo
Nov 5, 2013·Nature Chemical Biology·Ziyang HaoPeng R Chen

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Citations

Jan 26, 2016·Journal of Molecular Biology·Nilendra NairKelly Sheppard
Dec 18, 2015·Proteins·Thomas SimonsonPierre Plateau
Apr 6, 2012·PLoS Genetics·Alex M Plocik, Christine Guthrie
Dec 24, 2016·Molecular Microbiology·Richard J Lewis
Sep 30, 2016·Microbiology and Molecular Biology Reviews : MMBR·Daniel R ReußJörg Stülke
Jun 11, 2020·The Journal of Biological Chemistry·Rodney Tollerson, Michael Ibba
Mar 11, 2020·Frontiers in Microbiology·José Manuel Rubio-GómezAbdelali Daddaoua
May 26, 2016·EcoSal Plus·Richard Giegé, Mathias Springer

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