The Bacillus subtilis yqjI gene encodes the NADP+-dependent 6-P-gluconate dehydrogenase in the pentose phosphate pathway

Journal of Bacteriology
Nicola ZamboniUwe Sauer

Abstract

Despite the importance of the oxidative pentose phosphate (PP) pathway as a major source of reducing power and metabolic intermediates for biosynthetic processes, almost no direct genetic or biochemical evidence is available for Bacillus subtilis. Using a combination of knockout mutations in known and putative genes of the oxidative PP pathway and 13C-labeling experiments, we demonstrated that yqjI encodes the NADP+-dependent 6-P-gluconate dehydrogenase, as was hypothesized previously from sequence similarities. Moreover, YqjI was the predominant isoenzyme during glucose and gluconate catabolism, and its role in the oxidative PP pathway could not be played by either of two homologues, GntZ and YqeC. This conclusion is in contrast to the generally held view that GntZ is the relevant isoform; hence, we propose a new designation for yqjI, gndA, the monocistronic gene encoding the principal 6-P-gluconate dehydrogenase. Although we demonstrated the NAD+-dependent 6-P-gluconate dehydrogenase activity of GntZ, gntZ mutants exhibited no detectable phenotype on glucose, and GntZ did not contribute to PP pathway fluxes during growth on glucose. Since gntZ mutants grew normally on gluconate, the functional role of GntZ remains obscure, as...Continue Reading

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Citations

Sep 26, 2009·Applied Microbiology and Biotechnology·Yun Xia DuanXue Ming Zhao
Sep 19, 2012·Journal of Industrial Microbiology & Biotechnology·Gui-Hye Hwang, Jae-Yong Cho
Mar 23, 2005·FEMS Yeast Research·Lars M BlankUwe Sauer
Jan 12, 2010·Antioxidants & Redox Signaling·Lars M BlankBruno Bühler
Jun 22, 2006·Journal of Bacteriology·Guillaume LerondelStéphane Aymerich
Aug 1, 2012·Journal of Molecular Microbiology and Biotechnology·Natividad Cabrera-ValladaresGuillermo Gosset
Aug 27, 2005·BMC Bioinformatics·Nicola ZamboniUwe Sauer
Jan 10, 2014·Journal of Industrial Microbiology & Biotechnology·Gui-Hye Hwang, Jae-Yong Cho
Feb 11, 2015·Molecular Cell·Lior SinaiSigal Ben-Yehuda
Aug 9, 2015·Journal of Industrial Microbiology & Biotechnology·Meiyappan LakshmananDong-Yup Lee
Aug 19, 2015·Frontiers in Microbiology·Sebastiaan K SpaansServé W M Kengen
Aug 17, 2005·FEMS Microbiology Reviews·Uwe Sauer, Bernhard J Eikmanns
Aug 5, 2016·Microbiology·Bart R B Haverkorn van RijsewijkUwe Sauer
Dec 5, 2012·Angewandte Chemie·Manfred EggersdorferWerner Bonrath
Feb 23, 2020·Frontiers in Bioengineering and Biotechnology·Birthe HalmschlagLars M Blank
Jun 18, 2020·Journal of Bioscience and Bioengineering·Birthe HalmschlagLars Mathias Blank

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