The bacterial and archaeal community structures and methanogenic potential of the cecal microbiota of goats fed with hay and high-grain diets

Antonie van Leeuwenhoek
Wei JinWeiyun Zhu

Abstract

The cecum plays an important role in the feed fermentation of ruminants. However, information is very limited regarding the cecal microbiota and their methane production. In the present study, the cecal content from twelve local Chinese goats, fed with either a hay diet (0% grain) or a high-grain diet (71.5% grain), were used to investigate the bacterial and archaeal community and their methanogenic potential. Microbial community analysis was determined using high-throughput sequencing of 16S rRNA genes and real-time PCR, and the methanogenesis potential was assessed by in vitro fermentation with ground corn or hay as substrates. Compared with the hay group, the high-grain diet significantly increased the length and weight of the cecum, the proportions of starch and crude protein, the concentrations of volatile fatty acids and ammonia nitrogen, but decreased the pH values (P < 0.05). The high-grain diet significantly increased the abundances of bacteria and archaea (P < 0.05) and altered their community. For the bacterial community, the genera Bifidobacterium, Prevotella, and Treponema were significantly increased in the high-grain group (P < 0.05), while Akkermansia, Oscillospira, and Coprococcus were significantly decreased (...Continue Reading

References

Feb 1, 1976·Canadian Journal of Microbiology·C G TsaiG A Jones
Aug 1, 1985·European Journal of Immunology·J P Van de Merwe, J H Stegeman
Aug 1, 1985·Applied and Environmental Microbiology·B J Paster, E Canale-Parola
Nov 1, 1982·Journal of Dairy Science·H P MakkarS S Negi
Aug 26, 1999·Journal of Animal Science·C MartinB Michalet-Doreau
Sep 18, 2003·International Journal of Systematic and Evolutionary Microbiology·Kazuhiro YanagitaYoichi Kamagata
Sep 25, 2004·International Journal of Systematic and Evolutionary Microbiology·Muriel DerrienWillem M de Vos
Dec 1, 1981·Applied and Environmental Microbiology·T R PatelG A Jones
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Jun 28, 2011·Bioinformatics·Robert C EdgarRob Knight
Jun 28, 2011·Genome Biology·Nicola SegataCurtis Huttenhower
Dec 27, 2011·International Journal of Systematic and Evolutionary Microbiology·Mitsuo Sakamoto, Moriya Ohkuma
Mar 23, 2012·The ISME Journal·Clara Belzer, Willem M de Vos
Dec 18, 2012·Applied and Environmental Microbiology·M PopovaC Martin
Aug 27, 2013·Nature Biotechnology·Morgan G I LangilleCurtis Huttenhower
Nov 20, 2013·Gut Microbes·Jean-François BrugèreCorinne Malpuech-Brugère
Feb 6, 2014·PloS One·Pascale Blais LecoursCaroline Duchaine
Mar 29, 2014·Archaea : an International Microbiological Journal·Yong SuWeiyun Zhu
Mar 19, 2015·International Journal of Systematic and Evolutionary Microbiology·Aharon Oren, George M Garrity
Mar 22, 2016·Trends in Microbiology·Tom Konikoff, Uri Gophna
Apr 28, 2016·World Journal of Microbiology & Biotechnology·Meirong LiWeiyun Zhu
Sep 22, 2016·Veterinary World·G JayaprakashT Tamilmani

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Datasets Mentioned

BETA
SRP077000
SRP076852

Methods Mentioned

BETA
PCR
Assay

Software Mentioned

LEfSe
SPSS
PANDAseq
vegan
PICRUSt
R
ANOSIM
ea
utils
UCLUST

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