The bacterial enzyme RppH triggers messenger RNA degradation by 5' pyrophosphate removal.

Nature
Atilio DeanaJ G Belasco

Abstract

The long-standing assumption that messenger RNA (mRNA) degradation in Escherichia coli begins with endonucleolytic cleavage has been challenged by the recent discovery that RNA decay can be triggered by a prior non-nucleolytic event that marks transcripts for rapid turnover: the rate-determining conversion of the 5' terminus from a triphosphate to a monophosphate. This modification creates better substrates for the endonuclease RNase E, whose cleavage activity at internal sites is greatly enhanced when the RNA 5' end is monophosphorylated. Moreover, it suggests an explanation for the influence of 5' termini on the endonucleolytic cleavage of primary transcripts, which are triphosphorylated. However, no enzyme capable of removing pyrophosphate from RNA 5' ends has been identified in any bacterial species. Here we show that the E. coli protein RppH (formerly NudH/YgdP) is the RNA pyrophosphohydrolase that initiates mRNA decay by this 5'-end-dependent pathway. In vitro, RppH efficiently removes pyrophosphate from the 5' end of triphosphorylated RNA, irrespective of the identity of the 5'-terminal nucleotide. In vivo, it accelerates the degradation of hundreds of E. coli transcripts by converting their triphosphorylated 5' ends to ...Continue Reading

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Citations

Dec 17, 2015·Scientific Reports·Casey M DanielsAnthony K L Leung
Dec 30, 2008·Molecular Cell·Jamie Richards, Joel G Belasco
Jan 7, 2017·Molecular Cell·Joel G Belasco
Dec 10, 2016·Proteins·John R SroujiSteven E Brenner
Feb 23, 2017·Nature·Francesco NeriSalvatore Oliviero
Sep 2, 2017·FEMS Microbiology Letters·Umji ChoiChang-Ro Lee
Oct 17, 2017·Nucleic Acids Research·Lee-Ya ChuHanna S Yuan
Mar 29, 2018·BMC Genomics·Erin E GillFiona S L Brinkman
Apr 7, 2018·Transcription·Christina JuliusYulia Yuzenkova
Apr 6, 2018·RNA Biology·Daniel J LucianoJoel G Belasco
Jul 10, 2018·The Journal of General and Applied Microbiology·Satoshi EndoMasaaki Wachi
Feb 24, 2011·Wiley Interdisciplinary Reviews. RNA·Bijoy K Mohanty, Sidney R Kushner
Jul 4, 2018·Journal of Bacteriology·Amber B Sauder, Melissa M Kendall
Apr 15, 2014·Journal of Bacteriology·Kevin L HockettSteven E Lindow
Jan 15, 2014·Journal of Bacteriology·Patricia BralleyGeorge H Jones
Feb 3, 2016·Molecular and Cellular Biology·Yoshiko MaidaKenkichi Masutomi
Sep 18, 2012·Journal of Bacteriology·Daniel J LucianoJoel G Belasco
Oct 3, 2018·Wiley Interdisciplinary Reviews. RNA·Nikita VasilyevAlexander Serganov
Nov 4, 2008·Molecular Microbiology·Vanessa KhemiciAgamemnon J Carpousis
Nov 21, 2008·Molecular Microbiology·Patricia B Lodato, James B Kaper
Nov 22, 2018·Nucleic Acids Research·Taylor B UpdegroveGisela Storz
Aug 8, 2009·Molecular Microbiology·Jörg Vogel
Aug 18, 2009·Molecular Microbiology·Martin OvergaardPoul Valentin-Hansen
Nov 6, 2009·Molecular Microbiology·Louise KimeKenneth J McDowall
Mar 20, 2010·Chembiochem : a European Journal of Chemical Biology·Rafal TomeckiAndrzej Dziembowski
Jul 16, 2017·Biochemical Society Transactions·Tom Dendooven, Ben F Luisi

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