The bacteriophage kh receptor of Lactococcus lactis subsp. cremoris KH is the rhamnose of the extracellular wall polysaccharide.

Applied and Environmental Microbiology
R ValyaseviB L Geller

Abstract

A receptor for bacteriophages of lactic acid bacteria, including Lactococcus lactis subsp. cremoris KH, was found on the cell wall and not on the cell membrane, as determined by a phage-binding assay of sodium dodecyl sulfate- and mutanolysin-treated cell walls. The cell wall carbohydrates of L. lactis subsp. cremoris KH were analyzed by gas chromatography and mass spectrometry and found to contain rhamnose, galactose, glucose and N-acetylglucosamine. Similar analysis of mutants that were reduced in the ability to bind phages kh, 643, c2, ml3, and 1 indicated that galactose was essential for binding all phages. In addition, rhamnose was required for binding phages kh and ml3. Inhibition studies of phage binding by using two different lectins with a specificity for galactose indicated that phage kh may not bind directly to galactose. Rather, galactose may be an essential structural component located in the vicinity of the receptor. Incubation of any of the five phages with rhamnose or of phage kh with purified cell walls inactivated the phages. Inactivation required divalent cations and was irreversible. Inactivation of phages was stereospecific for rhamnose, as neither L-(+)- nor D-(-)-fucose (the stereoisomers of rhamnose) inh...Continue Reading

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Citations

Dec 1, 1995·Molecular Biotechnology·P K Dinsmore, T R Klaenhammer
Mar 17, 2009·Journal of Bacteriology·Marina SiponenValérie Campanacci
Jun 7, 2014·Frontiers in Microbiology·Marie-Pierre Chapot-Chartier
Aug 4, 2006·Journal of Bacteriology·Ausra GaidelyteDennis H Bamford
Mar 2, 2005·Journal of Dairy Science·B L GellerN Dunn
Sep 1, 1993·Journal of Bacteriology·B L GellerB Hettinger-Smith
Oct 7, 2004·Applied and Environmental Microbiology·Kitt DupontJytte Josephsen
Oct 7, 2004·Applied and Environmental Microbiology·Kitt DupontBirgitte Stuer-Lauridsen
Aug 5, 2004·Journal of Bacteriology·Liisa RäisänenTapani Alatossava
Aug 12, 2021·Computational and Structural Biotechnology Journal·Katherine LavelleJennifer Mahony

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