The Bacteroides fragilis pathogenicity island is contained in a putative novel conjugative transposon

Journal of Bacteriology
Augusto A Franco

Abstract

The genetic element flanking the Bacteroides fragilis pathogenicity island (BfPAI) in enterotoxigenic B. fragilis (ETBF) strain 86-5443-2-2 and a related genetic element in NCTC 9343 were characterized. The results suggested that these genetic elements are members of a new family of conjugative transposons (CTns) not described previously. These putative CTns, designated CTn86 and CTn9343 for ETBF 86-5443-2-2 and NCTC 9343, respectively, differ from previously described Bacteroides species CTns in a number of ways. These new transposons do not carry tetQ, and the excision from the chromosome to form a circular intermediate is not regulated by tetracycline; they are predicted to differ in their mechanism of transposition; and their sequences have very limited similarity with CTnDOT or other described CTns. CTn9343 is 64,229 bp in length, contains 61 potential open reading frames, and both ends contain IS21 transposases. Colony blot hybridization, PCR, and sequence analysis indicated that CTn86 has the same structure as CTn9343 except that CTn86 lacks a approximately 7-kb region containing truncated integrase (int2) and rteA genes and it contains the BfPAI integrated between the mob region and the bfmC gene. If these putative CTns...Continue Reading

References

May 25, 1991·Nucleic Acids Research·G G Wilson
Feb 1, 1989·Molecular & General Genetics : MGG·C ReimmannD Haas
Dec 1, 1987·Journal of Clinical Microbiology·L L MyersR B Sack
May 1, 1984·Infection and Immunity·L L MyersM M Border
Jun 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·C L SearsR L Van Tassell
Jan 1, 1995·Infection and Immunity·J S MoncriefT D Wilkins
Oct 1, 1993·Journal of Bacteriology·A M StevensA A Salyers
Jan 1, 1995·Scandinavian Journal of Infectious Diseases·V H San JoaquinC L Sears
Aug 1, 1996·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·L M Mundy, C L Sears
Mar 1, 1997·Infection and Immunity·A A FrancoC L Sears
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Oct 10, 1997·Nucleic Acids Research·C RudyG Churchward
Jul 11, 1998·Plasmid·C J SmithD P Bayley
Aug 4, 1998·Antimicrobial Agents and Chemotherapy·S MiyamaeF Yoshimura
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·J Mahillon, M Chandler
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·S WuC L Sears
Oct 26, 1999·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·G ZhangA Weintraub
Jun 27, 2000·Journal of Bacteriology·Q ChengA A Salyers
Apr 26, 2001·Plasmid·G BonheyoA A Salyers
Nov 16, 2001·Antimicrobial Agents and Chemotherapy·S MiyamaeH Nikaido
Aug 16, 2002·Molecular Microbiology·Augusto A FrancoCynthia L Sears
Jan 31, 2003·Gastroenterology·Shaoguang WuCynthia L Sears
Mar 29, 2003·Science·Jian XuJeffrey I Gordon
Aug 7, 2003·Applied and Environmental Microbiology·Yanping WangAbigail A Salyers
Jul 24, 2012·Journal of Bacteriology·Jillian L Waters, Abigail A Salyers

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Citations

Dec 29, 2011·Antimicrobial Agents and Chemotherapy·Yang LiuJianzhong Shen
Oct 31, 2006·Applied and Environmental Microbiology·Simy L BuckwoldAugusto A Franco
Oct 16, 2007·Clinical Microbiology Reviews·Hannah M Wexler
Apr 16, 2009·Clinical Microbiology Reviews·Cynthia L Sears
Aug 24, 2006·Infection and Immunity·Shaoguang WuCynthia L Sears
Apr 5, 2005·Journal of Bacteriology·M BacicC J Smith
Mar 29, 2013·Future Microbiology·Christine DejeaCynthia L Sears
Nov 8, 2008·Molecular Microbiology·Zoe HeatherAndrew S Waller
Jan 31, 2009·FEMS Microbiology Reviews·Mario JuhasDerrick W Crook
Aug 21, 2013·Mobile Genetic Elements·Mélanie V TrudelSteve J Charette
Dec 3, 2014·Anaerobe·Thomas Vognbjerg SydenhamUNKNOWN ESGAI (ESCMID Study Group on Anaerobic Infections)
Jun 28, 2016·PloS One·Jessica V Pierce, Harris D Bernstein
Dec 6, 2008·Memórias do Instituto Oswaldo Cruz·Karla R MirandaRegina M C P Domingues
Oct 14, 2006·Molecular Nutrition & Food Research·Robert Doug Wagner
Sep 26, 2007·Journal of Medical Microbiology·Gabriella TerhesElisabeth Nagy
Dec 11, 2020·PLoS Pathogens·Hannah C CarrowHiutung Chu
Dec 21, 2019·Journal of Molecular Biology·Ezequiel Valguarnera, Juliane Bubeck Wardenburg
Sep 1, 2021·Journal of Medical Microbiology·József SókiElisabeth Nagy

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