The baculovirus anti-apoptotic p35 protein promotes transformation of mouse embryo fibroblasts.

The Journal of Biological Chemistry
M ResnicoffR Baserga

Abstract

The baculovirus p35 protein is a potent inhibitor of programmed cell death induced by a variety of stimuli in insects, nematodes, and mammalian cell lines. The broad ability of p35 in preventing apoptosis has led us to investigate its effect on mouse embryo fibroblasts in vitro and in vivo. For this purpose, we have used R- cells (3T3-like fibroblasts derived from mouse embryos with a targeted disruption of the insulin-like growth factor I receptor (IGF-IR) genes) and R508 cells (derived from R- and with 15 x 10(3) IGF-IRs per cell). Both cell lines grow normally in monolayer, but they do not form colonies in soft agar, and they are non-tumorigenic in nude mice. We show here that, in addition to its anti-apoptotic effect, p35 causes transformation of R508 cells, as evidenced by the following: 1) decreased growth factor requirements, 2) ability to form foci in monolayer and colonies in soft agar, and 3) ability to form tumors in nude mice. Since R- cells stably transfected with p35 do not transform, our observations suggest that in addition to its effect as an inhibitor of apoptosis, the baculovirus p35 protein has transforming potential that requires the presence of the IGF-IR. The possibility that these two properties could be...Continue Reading

References

Jun 1, 1978·Nature·J Folkman, A Moscona
Jul 14, 1995·The Journal of Biological Chemistry·D R BeidlerV M Dixit
Aug 1, 1994·Molecular and Cellular Biology·R J Clem, L K Miller
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·D PragerS Melmed
Dec 1, 1993·Journal of Neurochemistry·S RabizadehD E Bredesen
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C SellR Baserga
Jan 1, 1994·The Journal of Cell Biology·J C Reed
Dec 13, 1996·The Journal of Biological Chemistry·J R SingletonE L Feldman
Mar 15, 1997·Genes & Development·S G KennedyN Hay
Jan 1, 1997·Vitamins and Hormones·R BasergaB Valentinis
Jul 1, 1997·Molecular and Cellular Biology·B ValentinisR Baserga
Nov 1, 1994·Trends in Cell Biology·G Nuñez, M F Clarke

❮ Previous
Next ❯

Citations

May 18, 2005·Biochemical and Biophysical Research Communications·Kenneth HollanderShimon Efrat
Oct 10, 2001·Biochimica Et Biophysica Acta·M ZörnigG Evan
Nov 5, 1999·Annual Review of Microbiology·A RoulstonP E Branton
Oct 25, 2016·Neurobiology of Disease·Jewel L PodratzAnthony Windebank
Jul 10, 1999·The Journal of Biological Chemistry·B B Wolf, D R Green
Oct 12, 2001·Annals of the New York Academy of Sciences·S J MeechR C Duke

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.