DOI: 10.1101/472571Nov 18, 2018Paper

The Barcode, UMI, Set format and BUStools

BioRxiv : the Preprint Server for Biology
Páll MelstedLior Pachter

Abstract

We introduce the Barcode-UMI-Set format (BUS) for representing pseudoalignments of reads from single-cell RNA-seq experiments. The format can be used with all single-cell RNA-seq technologies, and we show that BUS files can be efficiently generated. BUStools is a suite of tools for working with BUS files and facilitates rapid quantification and analysis of single-cell RNA-seq data. The BUS format therefore makes possible the development of modular, technology-specific, and robust workflows for single-cell RNA-seq analysis.

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Cell Separation
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Research Study
Analysis
Gene Feature
DNA Barcode, Taxonomic

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