PMID: 698214Sep 11, 1978Paper

The basic asymmetry of Na+-dependent glycine transport in Ehrlich cells

Biochimica Et Biophysica Acta
R M Johnstone

Abstract

Influx and efflux of glycine have been examined as a function of external and internal Na+ concentrations, respectively, when : formula: (see text) = O. With : formula: (see text) = O it was found that at comparable external and cellular Na+ levels, the Km for efflux was larger by an order of magnitude than the value for influx and the V for efflux was several times greater than the V for influx. For both fluxes the major effect of Na+ was to decrease the Km value. The observations are consistent with the conclusion that the Na+-dependent transport system is asymmetric per se. Influx and efflux of glycine were increased in a near linear manner by increasing the Na+ concentration from 13 to 100 mM, the half-time for glycine equilibration being a funcion of the Na+ concentration in absence of an electrochemical potential difference for Na+. In Na+-free media ([Na+] less than 5 mM) equilibration of glycine between cells and medium was not achieved after 60 min at 25 degrees C. With : formula: (see text) = O, efflux (or uptake) of glycine was not affected by internal (or external) K+ between 20 and 120 mM suggesting that K+ plays no direct role in Na+-dependent transport of glycine in Ehrlich cells.

References

Dec 1, 1975·Biochimica Et Biophysica Acta·R M Johnstone
Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J E Lever
Jun 1, 1977·Archives of Biochemistry and Biophysics·R R WhitesellD M Regen
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Jun 17, 1976·Biochimica Et Biophysica Acta·P C LarisR M Johnstone
Jan 1, 1974·The Journal of Membrane Biology·M Colombini, R M Johnstone
Feb 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·H Murer, U Hopfer
Jul 1, 1973·The Journal of Clinical Investigation·E R Batt, D Schachter
Aug 9, 1974·Biochimica Et Biophysica Acta·R M Johnstone
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Citations

Jan 1, 1985·The Journal of Membrane Biology·A M Weigensberg, R Blostein
Jan 1, 1985·Comparative Biochemistry and Physiology. A, Comparative Physiology·J Lerner
Jan 1, 1980·Biochimica Et Biophysica Acta·G J MerkelJ M Becker
Jul 1, 1980·Biochimica Et Biophysica Acta·R M Johnstone, P C Laris

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