The basophil activation marker defined by antibody 97A6 is identical to the ectonucleotide pyrophosphatase/phosphodiesterase 3.

Blood
Hans-Jörg BühringK Sano

Abstract

It has recently been shown that monoclonal antibody (MoAb) 97A6 detects a surface antigen expressed on basophils and their CD34(+) precursor cells, as well as the basophil cell line KU-812. In this report the partial amino acid sequence of affinity chromatography- and sodium dodecyl sulfate-polyacrylamide gel electrophoresis-separated 97A6 antigen(s) from KU-812 lysates was determined. Sequence alignment of high-performance liquid chromatography-selected tryptic peptides from the resulting 130- and 150-kd bands revealed a 100% identity with amino acids 393 to 405 of ectonucleotide pyrophosphatase/phosphodiesterase-3 (E-NPP3; CD203c) but not of the related ectoenzyme PC-1 (E-NPP1). Moreover, MoAb 97A6 selectively recognized 293 cells transfected with human E-NPP3, but did not react with cells transfected with PC-1 or parental 293 cells. In addition, E-NPP3 messenger RNA expression was detected in basophils but not other peripheral blood cells. Finally, MoAb 97A6 immunoprecipitated phosphodiesterase activity from KU-812 cells and peripheral blood basophils, but not from other cell populations. These data demonstrate that MoAb 97A6 recognizes the functionally active type II transmembrane ectoenzyme E-NPP3.

Citations

Jun 4, 2002·International Journal of Hematology·Peter ValentMichel Arock
Dec 31, 2004·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·S M ErdmannH F Merk
May 5, 2012·Purinergic Signalling·Herbert ZimmermannNorbert Sträter
Mar 15, 2002·Current Allergy and Asthma Reports·Donald MacGlashanJohn T Schroeder
Nov 29, 2012·Current Allergy and Asthma Reports·Emily C McGowan, Sarbjit Saini
Feb 14, 2004·Methods : a Companion to Methods in Enzymology·Peter ValentRudolf Valenta
Mar 8, 2002·Experimental Hematology·Ken TobaYoshifusa Aizawa
Dec 12, 2001·European Journal of Clinical Investigation·W FürederP Valent
Jul 14, 2006·International Archives of Allergy and Immunology·Jörg Kleine-TebbeBernhard F Gibbs
Jun 25, 2008·International Archives of Allergy and Immunology·Mizuho NagaoTakao Fujisawa
Oct 27, 2010·International Archives of Allergy and Immunology·Yael GernezKari C Nadeau
Feb 18, 2010·Clinical and Molecular Allergy : CMA·Susan MikkelsenHans Jürgen Hoffmann
Aug 5, 2014·Current Allergy and Asthma Reports·Hua Huang, Yapeng Li
Dec 21, 2010·Annual Review of Immunology·Hajime KarasuyamaTakeshi Wada
May 4, 2006·Current Opinion in Allergy and Clinical Immunology·Wayne G Shreffler
Sep 24, 2005·International Archives of Allergy and Immunology·Maria-Theresa KrauthPeter Valent
Dec 14, 2011·Expert Review of Clinical Immunology·Didier G EboWim J Stevens
Jul 3, 2015·Experimental and Therapeutic Medicine·Pei TangQingjun Pan
Oct 6, 2009·The Journal of Allergy and Clinical Immunology·Franco H FalconeDavid I Pritchard
Mar 6, 2007·The Journal of Allergy and Clinical Immunology·Laura M GoberSarbjit S Saini
Mar 10, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·D G EboW J Stevens
Jun 17, 2006·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K VasagarS S Saini
Apr 17, 2008·Cytometry. Part B, Clinical Cytometry·D G EboW J Stevens
Aug 22, 2006·Allergy·M Triggiani, G Marone
Nov 22, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J LinM J C Alcocer
Jan 19, 2008·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·B F GibbsF H Falcone
Jun 21, 2011·Immunological Reviews·John T Schroeder
May 17, 2011·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·F H FalconeB F Gibbs
Jul 19, 2012·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A ThyagarajanW G Shreffler
Jun 10, 2010·Cytometry. Part B, Clinical Cytometry·Eva M SturmGunter J Sturm
Aug 21, 2003·Biochemical and Biophysical Research Communications·Yoshihide OheTakashi Matozaki
Mar 9, 2006·The Journal of Allergy and Clinical Immunology·Douglas A PlagerKristin M Leiferman
Feb 13, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R BoumizaJ Bienvenu
Nov 18, 2014·Cytometry. Part B, Clinical Cytometry·Bernadette EberleinJeroen Buters
Apr 25, 2009·Allergology International : Official Journal of the Japanese Society of Allergology·Takao FujisawaMasao Yamaguchi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basophils

Basophils are myeloid cells with a high affinity IgE receptor and is involved in inflammatory responses during allergy. Discover the latest research on Basophils here.