The behavior of stem cells and progenitor cells in the periodontal ligament during wound healing as observed using immunohistochemical methods

Journal of Periodontal Research
S OhtaT Inoue

Abstract

The aim of this study was to identify stem cells or progenitor cells in the periodontal ligament and to investigate their behavior during wound healing of bone defects created experimentally in the alveolar process. Intradentinal cavities were created in the mesial root of the first molar of 25 adult male rats that were killed 1, 3, 5, 7 and 14 d after surgery. At each time-point, sections were stained immunohistochemically for CD44s (standard), CD34, c-KIT, PCNA, Cbfa-1 and 5-bromo-2-deoxyuridine using primary antibodies. For morphometric analysis, the ratios of Cbfa-1 and PCNA-positive cells were calculated from the total number of positive cells/10(4) microm(2) in the cavities. 5-Bromo-2-deoxyuridine-positive cells were observed in the periodontal ligament and had migrated into the wound areas. A small number of CD44s-, CD34- and c-KIT-positive cells were observed in the bone marrow, but none were observed in the periodontal ligament. CD44s-positive cells were only observed in the alveolar bone cavity at 5 d after surgery. CD34- and c-KIT-positive cells were only observed in the dentin cavity at 7 d after surgery. Cbfa-1 and PCNA scores tended to show an increase 7 d after surgery. Mesenchymal stem cells and hematopoietic st...Continue Reading

References

Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K Ikuta, I L Weissman
Jul 1, 1988·Science·G J SpangrudeI L Weissman
Mar 1, 1985·The Anatomical Record·C A McCulloch
Jan 1, 1980·Journal of Periodontal Research·T R GouldD M Brunette
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·R F PereiraD J Prockop
Jan 20, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S GronthosP J Simmons
Apr 2, 1999·Science·M F PittengerD R Marshak
Aug 24, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K StewartJ N Beresford
Nov 21, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·P Ducy
Apr 5, 2001·Nature·D OrlicP Anversa
Oct 10, 2001·The Journal of Pathology·R PoulsomN A Wright
Nov 29, 2001·The Journal of Cell Biology·J PrillerU Dirnagl
Dec 1, 2001·Journal of the American Society of Nephrology : JASN·Takahito ItoMasatsugu Hori
Jun 22, 2002·Nature·Yuehua JiangCatherine M Verfaillie
Sep 18, 2002·Kidney International·Sandeep GuptaMark E Rosenberg
Mar 21, 2003·The Annals of Thoracic Surgery·Sunil RangappaEugene Kwang Sim Wei
Apr 4, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Songtao Shi, Stan Gronthos
Jun 12, 2003·Annals of the New York Academy of Sciences·Donald Orlic
Jan 15, 2004·The Journal of Clinical Investigation·Naozumi HashimotoSem H Phan
Jul 10, 2004·Circulation Research·Mark F Pittenger, Bradley J Martin
Jul 30, 2004·American Journal of Respiratory and Critical Care Medicine·Shinji AbeStephen I Rennard
Nov 2, 2004·Journal of Periodontology·Hiroyuki KawaguchiHidemi Kurihara
Apr 9, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ilka OttRobert A J Oostendorp
Jun 1, 2006·Journal of Periodontology·Naohiko HasegawaHidemi Kurihara
Jul 11, 2006·Journal of Periodontal Research·K NagatomoI Ishikawa
Jul 26, 2006·Journal of Leukocyte Biology·Simone MeindlAdelheid Elbe-Bürger
Nov 2, 2006·Journal of Periodontal Research·S C ChenP M Bartold

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Citations

May 11, 2010·International Journal of Dentistry·Weibo Zhang, Pamela C Yelick
May 11, 2010·Journal of Periodontology·Tomas I MitranoAntonio Sanz
Dec 7, 2007·The Bulletin of Tokyo Dental College·Kenichi MatsuzakaTakashi Inoue
Aug 20, 2014·International Journal of Medical Sciences·Guo YeYaguang Weng
Jul 31, 2013·Journal of Periodontology·Daniel Fernando Pereira VasconcelosPedro Duarte Novaes
Oct 27, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Fa-Ming ChenMin Zhang
Dec 17, 2015·Journal of Oral Science·Hirotaka Sato, Yutaka Takaoka
Mar 7, 2013·Journal of Clinical Periodontology·Douglas P DickinsonUlf M E Wikesjö
May 17, 2012·Acta Odontologica Scandinavica·Emily Correna Carlo ReisTatiana Borges Carvalho
Jan 15, 2013·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Francesco G ManganoCarlo Mangano
Nov 5, 2014·Frontiers in Cell and Developmental Biology·Yasuyuki KimuraIkuo Morita
Nov 19, 2015·Connective Tissue Research·Jianru LiuYixiang Wang

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